Hydrogen peroxide (H₂O₂), as one of important reactive oxygen species (ROS), can be endogenously produced in the liver, and used as a biomarker for predicting drug-induced liver injury (DILI). So, it is important to construct an efficient method for monitoring H₂O₂ in the liver to assess DILI. Herein, a hepatocyte-targeted fluorescent probe (GCP) for ratiometric detection of H₂O₂ is developed. GCP consists of two main parts: coumarin-pyran-based fluorophore and galactose-based hepatocyte-targetable group. The probe itself shows near-infrared (NIR) emission owing to its extended π-conjugation. Meanwhile, a large emission shift from 706 to 482 nm (224 nm) is observed after the reaction with H₂O₂. Taking advantage of the spectroscopic property, GCP exhibits high sensitivity toward H₂O₂ with 19-fold fluorescence ratio enhancement and is highly selective over other ROS. Moreover, the probe possesses a good hepatocyte-targeting ability and is applied for ratiometric monitoring endogenous H₂O₂ in HepG2 cells. Furthermore, the probe can trace H₂O₂ level during DILI in HepG2 cells. Most importantly, GCP can locate in the liver of zebrafish and detect H₂O₂ level in the liver during DILI, which will be a potentially useful tool to assess DILI.

过氧化氢（H ₂ O ₂）作为重要的活性氧（ROS）之一，可以在肝脏中内源性产生，并用作预测药物诱发的肝损伤（DILI）的生物标记。因此，重要的是构建一种有效的监测肝脏中H ₂ O _2的方法以评估DILI。本文中，用于比例检测H ₂ O ₂的肝细胞靶向荧光探针（GCP）被开发。GCP由两个主要部分组成：基于香豆素-吡喃的荧光团和基于半乳糖的肝细胞靶向组。由于其扩展的π共轭，探针本身显示出近红外（NIR）发射。同时，在与H ₂ O ₂反应之后观察到从706nm到482nm（224nm）的大发射位移。利用光谱特性，GCP对H ₂ O ₂表现出高灵敏度，荧光比率提高了19倍，并且相对于其他ROS具有高度的选择性。此外，该探针具有良好的肝细胞靶向能力，可用于比例监测HepG2细胞内源性H ₂ O ₂。此外，探针可以追踪H ₂HepG2细胞中DILI期间的O ₂水平。最重要的是，GCP可以位于斑马鱼的肝脏中并在DILI期间检测肝脏中的H ₂ O ₂水平，这将是评估DILI的潜在有用工具。