Quorum quenching (QQ), the disruption of microbial communication, has proven to be effective as an innovative anti-biofouling strategy for membrane bioreactors (MBRs). However, QQ bacteria for anaerobic environments have not been extensively analyzed in previous research. This study thus investigated facultative QQ bacterial strains that exhibit potential for use in aerobic and anaerobic MBRs. Two novel QQ strains from the genus Pseudomonas (KS2 and KS10) were isolated from anaerobic digester sludge using signal molecules as the sole carbon source. The two QQ strains exhibited significant signal molecule degradation depending on the oxygen levels and demonstrated endogenous QQ activity, with KS2 producing lactonase and KS10 producing acylase. The QQ strains significantly reduced the formation of the biofilm generated by both Pseudomonas aeruginosa (PAO1) and real sludge. Facultative QQ strains have the potential to offer a more flexible option for effective biofouling control in both aerobic and anaerobic MBRs.

中文翻译：

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新型本地兼性群体猝灭细菌菌株的分离和表征，用于灵巧的生物结垢控制。

法定人数猝灭（QQ），即微生物交流的中断，已被证明可以有效地用作膜生物反应器（MBR）的一种创新的抗生物结垢策略。但是，在以前的研究中，没有对厌氧环境中的QQ细菌进行过广泛的分析。因此，这项研究调查了兼性QQ细菌菌株，它们在有氧和厌氧MBR中具有潜在的用途。使用信号分子作为唯一碳源，从厌氧消化池污泥中分离出两种来自假单胞菌属的新型QQ菌株（KS2和KS10）。这两个QQ菌株根据氧水平显示出明显的信号分子降解，并显示出内源QQ活性，其中KS2产生内酯酶，KS10产生酰基酶。QQ菌株显着减少了由铜绿假单胞菌（PAO1）和实际污泥产生的生物膜的形成。兼性QQ菌株有可能为有氧和厌氧MBR中的有效生物污染控制提供更灵活的选择。