In this study, we used a simple in-situ biomineralization method to immobilize Bacillus subtilis (B. subtilis)-derived laccase into the copper-Trimesic acid framework (Cu-BTC), and the synthesized Laccase@Cu-BTC particles were used to degrade tetracycline and ampicillin. Compared with free laccase, the Laccase@Cu-BTC showed 16.5-fold of activity recovery, higher thermo-tolerant performance, more excellent acid-proof ability and reusability. Without any mediators, Laccase@Cu-BTC displayed high degradation efficiency (nearly 100%) for tetracycline and ampicillin in some actual water. The degradation mechanism and proposed degradation pathways of tetracycline and ampicillin were discussed technically. Besides, bacteriostatic assay and survival test of Escherichia coli (E. coli) and B. subtilis confirmed the loss of antibiotic activity for tetracycline and ampicillin, as well as the low ecotoxicity of the degradation products. Our research demonstrates that Laccase@Cu-BTC has excellent performance in the effective removal of antibiotics and the detoxification of degradation products, which make it a promising candidate for environmental recovery.

在这项研究中，我们使用一种简单的原位生物矿化方法将枯草芽孢杆菌（B.subtilis）衍生的漆酶固定在铜-三苯甲基酸骨架（Cu-BTC）中，并使用合成的Laccase @ Cu-BTC颗粒降解四环素和氨苄青霉素。与游离漆酶相比，Laccase @ Cu-BTC具有16.5倍的活性恢复，更高的耐热性能，更优异的耐酸能力和可重复使用性。在没有任何介质的情况下，Laccase @ Cu-BTC在某些实际水中对四环素和氨苄青霉素的降解效率很高（接近100％）。从技术上讨论了四环素和氨苄青霉素的降解机理和拟议的降解途径。此外，大肠杆菌（E. coli）和枯草芽孢杆菌证实四环素和氨苄青霉素的抗生素活性丧失，降解产物的生态毒性低。我们的研究表明，Laccase @ Cu-BTC在有效去除抗生素和降解产物的解毒方面具有出色的性能，这使其成为恢复环境的有希望的候选者。