Circular RNAs (circRNAs) have been reported to regulate the gene expression through sponging corresponding microRNAs in multiple malignant tumors, including hepatocellular carcinoma (HCC). Up to now, the effects of circ_0001178 in HCC are barely known. In our current work, we tested circ_0001178 expression in HCC tissues and HCC cells and found it was greatly elevated. Then, we evaluated the function of circ_0001178 on HCC cell proliferation. We found HepG2 and Huh-7 cell proliferation was repressed after circ_0001178 shRNA was infected into the cells. Moreover, flow cytometry evidenced that HepG2 and Huh-7 cell apoptosis was markedly triggered and cell cycle was arrested. Meanwhile, it was shown that HCC cell migration and invasion capacity were markedly inhibited by loss of circ_0001178. Knockdown of circ_0001178 restrained HCC tumor growth in vivo. Then, miR-382 was predicted and confirmed as the target of circ_0001178. Circ_0001178 was demonstrated to modulate miR-382 expression negatively. The effect of circ_0001178 on HCC tumor was rescued by miR-382 overexpression. Furthermore, vascular epithelial growth factor A (VEGFA) is identified in various cancers. Currently, VEGFA was proved to be the downstream target of miR-382. To conclude, this research revealed that circ_0001178 induced HCC progression via modulating miR-382 and VEGFA axis.

中文翻译：

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Circ_0001178 调节 miR-382/VEGFA 轴以促进肝细胞癌进展。

据报道，环状 RNA (circRNAs) 通过在包括肝细胞癌 (HCC) 在内的多种恶性肿瘤中吸收相应的 microRNA 来调节基因表达。到目前为止，circ_0001178 在 HCC 中的作用还鲜为人知。在我们目前的工作中，我们测试了 circ_0001178 在 HCC 组织和 HCC 细胞中的表达，发现它大大升高。然后，我们评估了 circ_0001178 对 HCC 细胞增殖的作用。我们发现在 circ_0001178 shRNA 感染细胞后，HepG2 和 Huh-7 细胞增殖受到抑制。此外，流式细胞术证明 HepG2 和 Huh-7 细胞凋亡被显着触发，细胞周期被阻止。同时，表明 circ_0001178 的缺失显着抑制了 HCC 细胞的迁移和侵袭能力。circ_0001178 的敲低抑制了体内 HCC 肿瘤的生长。然后，miR-382被预测并确认为circ_0001178的靶标。Circ_0001178 被证明可以负向调节 miR-382 的表达。miR-382过表达挽救了circ_0001178对HCC肿瘤的影响。此外，血管上皮生长因子 A (VEGFA) 在各种癌症中得到鉴定。目前，VEGFA 被证明是 miR-382 的下游靶点。总之，这项研究表明，circ_0001178 通过调节 miR-382 和 VEGFA 轴诱导 HCC 进展。血管上皮生长因子 A (VEGFA) 在各种癌症中得到鉴定。目前，VEGFA 被证明是 miR-382 的下游靶点。总之，这项研究表明，circ_0001178 通过调节 miR-382 和 VEGFA 轴诱导 HCC 进展。血管上皮生长因子 A (VEGFA) 在各种癌症中得到鉴定。目前，VEGFA 被证明是 miR-382 的下游靶点。总之，这项研究表明，circ_0001178 通过调节 miR-382 和 VEGFA 轴诱导 HCC 进展。