Biochar has proven to be a feasible additive for mitigating nitrogen loss during the composting process. This study aims to evaluate the influence of biochar addition on bacterial community and physicochemical properties changes, including ammonium (NH₄⁺), nitrite (NO₂⁻) and nitrate (NO₃⁻) contents during the composting of poultry manure. The composting was carried out by adding 20% (w/w) of biochar into the mixture of poultry manure and rice straw with a ratio of 2:1, and the same treatment without biochar was prepared as a control. The finished product of control compost recorded the high contents of NO₂⁻ and NO₃⁻ (366 mg/kg and 600 mg/kg) with reduced the total NH₄⁺ content to 10 mg/kg. Meanwhile, biochar compost recorded a higher amount of total NH₄⁺ content (110 mg/kg) with low NO₂⁻ and NO₃⁻ (161 mg/kg and 137 mg/kg) content in the final composting material. The principal component analysis showed that the dynamics of dominant genera related to Halomonas, Pusillimonas, and Pseudofulvimonas, all of which were known as nitrifying and denitrifying bacteria, was significantly correlated with the dynamic of NO₂⁻ and NO₃⁻ content throughout the composting process. The genera related to Pusillimonas, and Pseudofulvimonas appeared as the dominant communities as the NO₂⁻ and NO₃⁻ increased. In contrast, as the NO₂⁻ and NO₃⁻ concentration decreased, the Halomonas genus were notably enriched in biochar compost. This study revealed the bacterial community shifts corresponded with the change of physicochemical properties, which provides essential information for a better understanding of monitoring and improving the composting process.

事实证明，生物炭是减轻堆肥过程中氮损失的可行添加剂。本研究旨在评估生物炭添加对细菌群落和物理化学性质的变化，包括铵的影响（NH ₄ ⁺），亚硝酸根（NO ₂ ^- ）和硝酸（NO ₃ ^- ）家禽粪便的堆肥过程中的内容。将20％（w / w）的生物炭以2：1的比例添加到家禽粪便和稻草的混合物中进行堆肥，并准备无生物炭的相同处理作为对照。控制堆肥的成品记录NO的含量高₂ ^-和NO ₃ ^-（366 mg / kg和600 mg / kg），总NH ₄ ⁺含量降至10 mg / kg。同时，生物炭堆肥记录更大量的总的NH ₄ ⁺含量（110毫克/千克）与低NO ₂ ^-和NO ₃ ^-（161 mg / kg和137毫克/千克）在最终的堆肥材料的内容。主成分分析表明，与主导属的动力学盐单胞菌，Pusillimonas，和Pseudofulvimonas，所有这些被称为硝化和反硝化细菌，是与显著NO：1的动态相关₂ ^-和NO ₃ ^-在堆肥过程中的含量。该属有关Pusillimonas，并Pseudofulvimonas出现为主导的社区为NO ₂ ^-和NO ₃ ^-增加。与此相反，作为NO ₂ ^-和NO ₃ ^-的浓度降低时，盐单胞菌属中特别是在生物炭堆肥富集。这项研究揭示了细菌群落的变化与理化性质的变化相对应，这为更好地了解监测和改善堆肥过程提供了重要信息。