Jug r 1, the major allergen of walnut, triggers severe allergic reactions through epitopes. Hence, research on the efficient strategy for analyzing the linear epitopes of Jug r 1 are necessary. In this work, bioinformatics analysis was used to predict the linear epitopes of Jug r 1. Overlapping peptide synthesis was used to map linear epitopes. In vitro simulated gastrointestinal digestion and HPLC-MS/MS were used to identify digestion-resistant peptides. The results showed that six predicted linear epitopes were AA28-35, AA42-49, AA55-62, AA65-73, AA97-104, and AA109-121. AA16-30 and AA125-139 were identified by the sera of walnut allergic patients. Five digestion-resistant peptides were AA19-33, AA40-45, AA54-74, AA96-106, and AA117-137. The predicted results only included one of the linear epitopes identified by sera, while the digestion-resistant peptides covered all. Therefore, the digestion-resistant property of food allergens may be a promising direction for studying the linear epitopes of Jug r 1.

中文翻译：

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抗消化肽的序列分析可能是研究核桃主要变应原Jug r 1线性表位的有效策略。

核桃的主要过敏原Jug r 1通过表位引发严重的过敏反应。因此，有必要研究一种有效的策略来分析Jug r 1的线性表位。在这项工作中，生物信息学分析被用来预测Jug r 1的线性表位。重叠的肽合成被用来绘制线性表位。体外模拟胃肠消化和HPLC-MS / MS用于鉴定抗消化肽。结果表明，六个预测的线性表位是AA28-35，AA42-49，AA55-62，AA65-73，AA97-104和AA109-121。通过核桃过敏患者的血清鉴定出AA16-30和AA125-139。五个抗消化肽是AA19-33，AA40-45，AA54-74，AA96-106和AA117-137。预测结果仅包括由血清鉴定的线性表位之一，而抗消化肽覆盖了所有。因此，食物过敏原的抗消化特性可能是研究Jug r 1线性表位的有前途的方向。