Immobilized liposome chromatography (ILC) is a powerful tool in screening and analyzing membrane-permeable components in natural medicinal herbs. In this study, the stationary phase of a new receptor liposome biomembrane chromatography (RLBC) was employed to screen the active ingredients in seven natural medicinal herbs. As a model system, α-glucosidase was immobilized in porous silica gel by incorporating α-glucosidase into liposome vesicles. Combined with HPLC, this stationary phase can be used to evaluate the interaction of liposome-protein compounds with compounds and drugs. The surface characteristics of the RLBC phase was characterized by Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and Energy dispersive spectrometer (EDS). RLBC was successfully used to screen and analyze permeable compounds in natural medicinal herbs, and screen the extracts from Schisandra chinensis. This method was used to establish that Schisandrin in Schisandra chinensis is an active component. Furthermore, the hypoglycemic effect of Schisandrin was verified in vivo in rats. This study further modeled the relationship between the activity of inhibitor and retention behavior with RLBC with a mathematical equation. RLBC stationary phase combined with HPLC can be used for rapid screening of drug candidates.

中文翻译：

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开发用于筛选和表征 α-葡萄糖苷酶结合化合物的固定化脂质体色谱方法。


固定化脂质体色谱（ILC）是筛选和分析天然草药中膜渗透成分的有力工具。本研究采用新型受体脂质体生物膜色谱（RLBC）固定相筛选七种天然药材的活性成分。作为模型系统，通过将α-葡萄糖苷酶掺入脂质体囊泡中，将α-葡萄糖苷酶固定在多孔硅胶中。与 HPLC 结合，该固定相可用于评估脂质体-蛋白质化合物与化合物和药物的相互作用。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）和能谱仪（EDS）对RLBC相的表面特征进行了表征。 RLBC成功用于天然药材中渗透性化合物的筛选和分析，并筛选了五味子提取物。该方法用于确定五味子中的五味子素是活性成分。此外，五味子素的降血糖作用在大鼠体内得到了验证。本研究进一步用数学方程模拟了抑制剂活性与 RLBC 保留行为之间的关系。 RLBC固定相结合HPLC可用于快速筛选候选药物。