Determination of busulfan concentration in patients undergoing bone marrow transplantation is necessary in order to reduce toxic effects and/or graft rejection due to unadjusted dose exposure. A new extraction method namely dispersive micro solid phase extraction (DMSPE) based on mesoporous sorbent was used for cleaning-up the plasma samples. DMSPE coupling with liquid chromatography with tandem mass spectrometry (LC-MS/MS) was implemented for the determination of busulfan dosage in plasma samples. The linear range was found from 10 to 2000 ng/ml. The precision and accuracy were found better than 15% according to Food and drug Administration (FDA) guideline. This method was successfully used to determine the busulfan in patients administrated busulfan as part of the preparative regimen for bone marrow transplantation.

中文翻译：

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在通过HPLC-MS / MS测定之前，使用新型介孔吸附剂从血浆样品中进行分散体微固相萃取白硫丹。

必须确定接受骨髓移植的患者中的白消安浓度，以减少由于未经调整的剂量暴露引起的毒性作用和/或移植排斥。一种新的提取方法，即基于中孔吸附剂的分散微固相提取（DMSPE）用于净化血浆样品。DMSPE与液相色谱-串联质谱联用（LC-MS / MS）用于测定血浆样品中的白消安剂量。发现线性范围为10至2000ng / ml。根据美国食品药品监督管理局（FDA）的指南，发现精度和准确度优于15％。该方法已成功地用于确定使用白消安的患者中的白消安，作为骨髓移植准备方案的一部分。