Alternaria mycotoxins, such as tenuazonic acid (TeA), altenuene (ALT), alternariol (AOH), tentoxin (TEN) and alternariol monomethyl ether (AME) are frequently found in foods and may pose a potential risk to human health. Human biomonitoring can help measure our exposure to these mycotoxins, and help us determine if the exposure is changing over time. In this study, a simple liquid-liquid extraction sample preparation procedure followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed for the simultaneous analysis of five Alternaria mycotoxins in human urine. High recoveries (92.7-103.2%) were obtained for all the tested mycotoxins with relative standard deviations (RSDs, %) of less than 6.4%. The limits of quantification (LOQs) for the analytes in urine ranged from 0.001 to 0.05 ng/mL. The method was successfully applied to investigate the levels of five Alternaria mycotoxins from 135 volunteers. In all of the samples, at least one Alternaria mycotoxin was detected. TeA, AME and AOH were the predominant Alternaria mycotoxins, and the detection rates were 85.9%, 96.3% and 51.9%, respectively.

中文翻译：

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开发一种简单而快速的LC-MS / MS方法，用于同时定量人尿中的五种链霉菌真菌毒素。

食物中经常发现链霉菌霉菌毒素，如替纳唑酸（TeA），altenuene（ALT），链烷醇（AOH），腱毒素（TEN）和链烷醇单甲醚（AME），可能对人类健康构成潜在风险。人体生物监测可以帮助我们评估这些真菌毒素的暴露量，并帮助我们确定暴露量是否随时间变化。在这项研究中，开发了一种简单的液-液萃取样品制备程序，然后将液相色谱与串联质谱联用（LC-MS / MS），用于同时分析人尿中的五种链霉菌真菌毒素。所有测试的霉菌毒素均具有较高的回收率（92.7-103.2％），相对标准偏差（RSDs，％）小于6.4％。尿液中分析物的定量限（LOQ）为0.001至0.05 ng / mL。该方法已成功应用于调查135名志愿者中5种链霉菌真菌毒素的水平。在所有样品中，至少检测到一种链格孢菌毒素。TeA，AME和AOH是主要的链霉菌真菌毒素，检出率分别为85.9％，96.3％和51.9％。