Background

This study aimed to test the hypothesis that long non-coding RNA (lncRNA) colorectal neoplasia differentially expressed (CRNDE) could exacerbate brain injury caused by intrauterine infection in neonatal rats.

Methods

Intrauterine infection was induced in pregnant rats by lipopolysaccharide (LPS). After delivery, newborn rats with brain injury caused by intrauterine infection were randomly divided into control, control shRNA, and CRNDE shRNA groups. CRNDE expression in serum and amniotic fluid of pregnant rats and neonatal brain tissues were determined by quantitative real-time PCR (qRT-PCR). Morris water maze (MWM) task was used to test the spatial learning and memory ability. Histological examination and apoptosis detection were performed by hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, respectively. Immunohistochemistry was conducted to evaluate the activation of astrocytes and microglia.

Results

LncRNA CRNDE was highly expressed in serum and amniotic fluid of maternal rats and in brain tissues of offspring rats. Furthermore, shRNA-mediated CRNDE downregulation could rescue the spatial learning and memory ability, improve brain histopathological changes and cell death, and inhibit the activation of astrocytes and microglia caused by LPS.

Conclusion

CRNDE silencing possessed a cerebral protective effect in neonatal rats with brain injury caused by interauterine infection.

中文翻译：

---

较长的非编码RNA CRNDE使子宫内感染引起的新生儿脑损伤恶化。

背景

这项研究旨在检验以下假设，即长时非编码RNA（lncRNA）结肠直肠癌的差异表达（CRNDE）可能加剧新生大鼠子宫内感染引起的脑损伤。

方法

脂多糖（LPS）在怀孕的大鼠中引起宫内感染。分娩后，将因宫内感染引起的脑损伤新生大鼠随机分为对照组，对照组shRNA和CRNDE shRNA组。通过定量实时荧光定量PCR（qRT-PCR）测定妊娠大鼠血清和羊水和新生儿脑组织中CRNDE的表达。莫里斯水迷宫（MWM）任务用于测试空间学习和记忆能力。分别通过苏木精和曙红（H＆E）和末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）染色进行组织学检查和凋亡检测。进行了免疫组织化学以评估星形胶质细胞和小胶质细胞的活化。

结果

LncRNA CRNDE在雌性大鼠的血清和羊水以及后代大鼠的脑组织中高表达。此外，shRNA介导的CRNDE下调可以挽救空间学习和记忆能力，改善脑组织病理学变化和细胞死亡，并抑制LPS引起的星形胶质细胞和小胶质细胞的活化。

结论

CRNDE沉默在新生小鼠中因宫外感染引起的脑损伤具有脑保护作用。