UV light-emitting diodes (UV-LEDs) have emerged as a new technology for water disinfection. Multiwell plates are a common tool in biological research, but they have never been used for UVC/UVB-inactivation experiments of microorganisms. In this study, a novel, rapid and simple UVC/UVB-inactivation assay was developed for a UV-LED system using a multiwell plate setup (96- and 24-well plates). The relative incident irradiance distribution across the exposed area was examined by spectroradiometry and nitrate-nitrite uniformity assay. The two methods showed a good correlation and high distribution factors (>0.89 and >0.94 for 96- and 24-well plates, respectively). In addition, the potential of the new system for determining disinfection efficacy of E. coli and MS2 coliphage by UV-LEDs emitting at central wavelengths of 265 nm and 285 nm was demonstrated. The inactivation rate constants were comparable to those obtained using UV-LED systems with the conventional dish (or beaker) setup, but the multiwell plate method allowed for many more repetitions. The proposed system is an alternative for UV-inactivation dose-response assay, especially when screening assays are desired, since it has the advantage of being fast, comprehensive (with a large number of simultaneous replicates) and easily adapted to various applications as UV-LED based photocatalysis experiments, UV effect on biofilm formation and UV-based AOP degradation experiments.

中文翻译：

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用于在UV-LED系统中获得快速微生物剂量响应曲线的多孔板。

紫外线发光二极管（UV-LEDs）已经成为一种用于水消毒的新技术。多孔板是生物学研究中的常用工具，但从未用于微生物的UVC / UVB灭活实验。在这项研究中，为使用多孔板设置（96孔板和24孔板）的UV-LED系统开发了一种新颖，快速，简单的UVC / UVB灭活测定法。通过分光光度法和硝酸盐-亚硝酸盐均匀性测定法检查了整个曝光区域的相对入射辐照度分布。两种方法显示出良好的相关性和较高的分布因子（96孔板和24孔板分别> 0.89和> 0.94）。此外，新系统具有确定E.消毒功效的潜力。通过在265nm和285nm的中心波长发射的UV-LED证实了大肠杆菌和MS2大肠杆菌噬菌体。灭活速率常数可与使用具有常规培养皿（或烧杯）设置的UV-LED系统获得的常数相比，但是多孔板方法可进行更多重复。拟议的系统是紫外线灭活剂量反应测定的替代方法，尤其是在需要筛选测定时，因为它具有快速，全面（具有大量同时重复）的优点，并且易于适应各种应用，例如紫外线基于LED的光催化实验，紫外线对生物膜形成的影响以及基于紫外线的AOP降解实验。但是多孔板方法允许更多重复。拟议的系统是紫外线灭活剂量反应测定的替代方法，尤其是在需要筛选测定时，因为它具有快速，全面（具有大量同时重复）的优点，并且易于适应各种应用，例如紫外线基于LED的光催化实验，紫外线对生物膜形成的影响以及基于紫外线的AOP降解实验。但是多孔板方法允许更多重复。拟议的系统是紫外线灭活剂量反应测定的替代方法，尤其是在需要筛选测定时，因为它具有快速，全面（具有大量同时重复）的优点，并且易于适应各种应用，例如紫外线基于LED的光催化实验，紫外线对生物膜形成的影响以及基于紫外线的AOP降解实验。