Abstract In this study, secondary brewery wastewater (SBWW) supplemented with sugarcane molasses (SCM) was used for SBWW treatment with concomitant lipid and carotenoid production by the yeast Rhodosporidium toruloides NCYC 921. In order to improve the biomass production, ammonium sulfate, yeast extract and urea were tested as nitrogen sources. Urea was chosen as the best low-cost nitrogen source. A fed-batch cultivation was carried out with SBWW supplemented with 10 g L−1 of sugarcane molasses as carbon source, and 2 g L−1 of urea as nitrogen source. A maximum biomass concentration of 42.5 g L−1 was obtained at t = 126.5 h and the maximum biomass productivity was 0.55 g L−1 h−1 at t = 48.25 h. The maximum lipid content was 29.9 % w/w (DCW) at t = 94 h of cultivation and the maximum carotenoid content was 0.23 mg g−1 at 120 h of cultivation. Relatively to the SBWW treatment, after the batch phase, 45.8 % of total Kjeldahl nitrogen removal, 81.7 % of COD removal and 100 % of sugar consumption were observed. Flow cytometry analysis revealed that 27.27 % of the cells had injured membrane after the inoculation. This proportion was reduced to 10.37 % at the end of the cultivation, indicating that cells adapted to the growth conditions.

中文翻译：

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用富含甘蔗糖蜜和尿素的啤酒废水中生长的产油酵母 Rhodosporidium toruloides 生产脂质和类胡萝卜素的伴随废水处理

摘要 本研究以啤酒厂二级废水 (SBWW) 为辅的甘蔗糖浆 (SCM) 用于 SBWW 处理，同时酵母 Rhodosporidium toruloides NCYC 921 产生脂质和类胡萝卜素。为了提高生物质产量，硫酸铵、酵母提取物和尿素作为氮源进行测试。尿素被选为最佳的低成本氮源。SBWW补料分批培养，补充10 g L-1甘蔗糖蜜作为碳源，2 g L-1尿素作为氮源。在 t = 126.5 h 时获得了 42.5 g L-1 的最大生物量浓度，在 t = 48.25 h 时获得的最大生物量生产力为 0.55 g L-1 h-1。在 t = 94 小时培养时，最大脂质含量为 29.9 % w/w (DCW)，在培养 120 小时时最大类胡萝卜素含量为 0.23 mg g-1。相对于 SBWW 处理，在分批阶段后，观察到 45.8% 的凯氏定氮去除总量、81.7% 的 COD 去除率和 100% 的糖消耗量。流式细胞术分析显示27.27%的细胞在接种后膜受损。该比例在培养结束时降至 10.37%，表明细胞适应了生长条件。