Glyphosate-tolerant soybean is the most widely planted genetically modified crop worldwide. However, soybean remains recalcitrant to routine transformation because of the low infection efficiency of Agrobacterium to soybean and lack of useful selectable markers. In this study, several Agrobacterium strains and cell densities were compared by transient expression of the GUS gene. The results showed that Agrobacterium strain Ag10 at cell densities of OD₆₀₀ of 0.6–0.9 yielded the highest infection efficiency in Agrobacterium-mediated soybean cotyledonary node transformation system. Meanwhile, a simple and rapid method was developed for identification of glyphosate tolerance in putative T₀ transgenic plants, consisting of spotting plantlets with 1 µL Roundup®. The whole cycle of genetic transformation could be shortened to about 3 mon by highly efficient selection with glyphosate during the transformation process and application of the spot assay in putative T₀ transgenic plantlets. The transformation frequency ranged from 2.9 to 5.6%. This study provides an improved protocol for development and identification of glyphosate-tolerant transgenic soybeans.

耐草甘膦的大豆是全世界种植最广泛的转基因作物。然而，由于农杆菌对大豆的感染效率低和缺乏有用的选择标记，大豆仍然对常规转化不利。在这项研究中，通过瞬时表达GUS基因比较了几种农杆菌菌株和细胞密度。结果表明，农杆菌菌株Ag10在OD ₆₀₀的细胞密度为0.6-0.9时在农杆菌中产生最高的感染效率。介导的大豆子叶节转化系统。同时，开发了一种简单快速的方法来鉴定推定的T ₀转基因植物中的草甘膦耐受性，该方法包括用1 µLRoundup®斑点苗。通过在转化过程中使用草甘膦进行高效选择以及在假定的T ₀转基因小植株中进行斑点检测，可以将草甘膦的高效基因选择转化为整个基因转化的周期缩短至约三个月。转换频率范围从2.9到5.6％。这项研究为耐草甘膦转基因大豆的开发和鉴定提供了改进的方案。