The digestive tract of termite (Microcerotermes diversus) contains a variety of lignocellulose-degrading bacteria with exocellulases enzyme activity, not found in the rumen, which could potentially improve fiber degradation in the rumen. The objectives of the current study were to determine the effect of inoculation of rumen fluid (RF) with three species of bacteria isolated from termite digestive tract, Bacillus licheniformis, Ochrobactrum intermedium, and Microbacterium paludicola, on in vitro gas production (IVGP), fermentation parameters, nutrient disappearance, microbial populations, and hydrolytic enzyme activities with fibrous wheat straw (WS) and date leaf (DL) as incubation substrate. Inoculation of RF with either of three termite bacteria increased (P<0.05) ammonia-N concentration compared with the control group (free of termite gut bacteria). Termite bacteria inoculation had no effect (P>0.05) on gas production characteristics, dry matter, organic matter and neutral detergent fiber disappearance, pH, and concentration and composition of volatile fatty acids. Population of proteolytic bacteria and protozoa, but not cellulolytic bacteria, were increased (P<0.05) when RF was inoculated with termite bacteria with both WS and DL substrates. Inoculation of RF with termite bacteria increased protease activity, while activities of carboxymethyl-cellulase, microcrystalline-cellulase, α-amylase and filter paper degrading activity remained unchanged (P>0.05). Overall, the results of this study indicated that transferring lignocellulose-degrading bacteria, isolated from digestive tract of termite, to rumen liquid increased protozoa and proteolytic bacteria population and consequently increased protease activity and ammonia-N concentration in vitro, however, no effect on fermentation and fiber degradation parameters were detected. These results suggest that the termite bacteria might be rapidly lysed by the rumen microbes before beneficial effects on the rumen fermentation process could occur.

白蚁的消化道（Microcerotermes diversus）含有多种降解木质素纤维素的细菌，具有瘤胃中不存在的纤维素酶活性，这可能会改善瘤胃中纤维的降解。本研究的目的是确定从白蚁消化道，地衣芽孢杆菌，中间ch骨和中型微细菌中分离出的三种细菌接种瘤胃液（RF）对体外的影响气体产量（IVGP），发酵参数，养分消失，微生物种群和水解酶活性，以纤维麦草（WS）和枣叶（DL）为孵化底物。与对照组（不含白蚁肠道细菌）相比，用三种白蚁细菌中的任一种接种RF均可增加（P <0.05）氨氮浓度。接种白蚁细菌对产气特性，干物质，有机物质和中性洗涤剂纤维的消失，pH以及挥发性脂肪酸的浓度和组成没有影响（P > 0.05）。蛋白水解细菌和原生动物的数量增加了，但纤维素分解细菌却没有增加（P<0.05）当RF接种带有WS和DL底物的白蚁细菌时。用白蚁菌接种RF可增加蛋白酶活性，而羧甲基纤维素酶，微晶纤维素酶，α-淀粉酶和滤纸的降解活性保持不变（P > 0.05）。总体而言，这项研究的结果表明，将从白蚁消化道分离出的降解木质纤维素的细菌转移到瘤胃液中，增加了原生动物和蛋白水解细菌的种群，从而增加了体外的蛋白酶活性和氨氮浓度，但是，未检测到对发酵和纤维降解参数的影响。这些结果表明，在对瘤胃发酵过程产生有益影响之前，白蚁细菌可能会被瘤胃微生物迅速溶解。