OS is the most common malignant tumor of bone which was featured with osteoid or immature bone produced by the malignant cells, and biomarkers are urgently needed to identify patients with this aggressive disease. We downloaded gene expression profiles from GEO and TARGET datasets for OS, respectively, and performed WGCNA to identify the key module. Whereafter, functional annotation and GSEA demonstrated the relationships between target genes and OS. In this study, we discovered four key genes—ALOX5AP, HLA-DMB, HLA-DRA and SPINT2 as new prognostic markers and confirmed their relationship with OS metastasis in the validation set. In conclusion, ALOX5AP, HLA-DMB, HLA-DRA and SPINT2 were identified by bioinformatics analysis as possible prognostic markers for OS metastasis.

中文翻译：

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表达谱分析将关键基因确定为骨肉瘤转移的预后标志物

OS是最常见的骨恶性肿瘤，以恶性细胞产生的类骨质或未成熟骨为特征，迫切需要生物标志物来识别患有这种侵袭性疾病的患者。我们分别从 GEO 和 TARGET 数据集中下载了 OS 的基因表达谱，并执行 WGCNA 来识别关键模块。此后，功能注释和GSEA证明了靶基因与OS之间的关系。在这项研究中，我们发现了四个关键基因——ALOX5AP、HLA-DMB、HLA-DRA 和 SPINT2 作为新的预后标志物，并在验证集中证实了它们与 OS 转移的关系。总之，通过生物信息学分析，ALOX5AP、HLA-DMB、HLA-DRA 和 SPINT2 被鉴定为 OS 转移的可能预后标志物。