The non-climacteric ‘Yellow’ melon (Cucumis melo, inodorus group) is an economically important crop and its quality is mainly determined by the sugar content. Thus, knowledge of sugar metabolism and its related pathways can contribute to the development of new field management and post-harvest practices, making it possible to deliver better quality fruits to consumers. The RNA-seq associated with RT-qPCR analyses of four maturation stages were performed to identify important enzymes and pathways that are involved in the ripening profile of non-climacteric ‘Yellow’ melon fruit focusing on sugar metabolism. We identified 895 genes 10 days after pollination (DAP)-biased and 909 genes 40 DAP-biased. The KEGG pathway enrichment analysis of these differentially expressed (DE) genes revealed that ‘hormone signal transduction’, ‘carbon metabolism’, ‘sucrose metabolism’, ‘protein processing in endoplasmic reticulum’ and ‘spliceosome’ were the most differentially regulated processes occurring during melon development. In the sucrose metabolism, five DE genes are up-regulated and 12 are down-regulated during fruit ripening. The results demonstrated important enzymes in the sugar pathway that are responsible for the sucrose content and maturation profile in non-climacteric ‘Yellow’ melon. New DE genes were first detected for melon in this study such as invertase inhibitor LIKE 3 (CmINH3), trehalose phosphate phosphatase (CmTPP1) and trehalose phosphate synthases (CmTPS5, CmTPS7, CmTPS9). Furthermore, the results of the protein-protein network interaction demonstrated general characteristics of the transcriptome of young and full-ripe melon and provide new perspectives for the understanding of ripening.

中文翻译：

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非更年期“黄”瓜成熟过程中的转录组分析：糖代谢的见解


非更年期“黄”甜瓜（Cucumis melo，inodorus group）是一种重要的经济作物，其品质主要取决于糖含量。因此，糖代谢及其相关途径的知识有助于开发新的田间管理和采后实践，从而可以为消费者提供更优质的水果。进行了与四个成熟阶段的 RT-qPCR 分析相关的 RNA-seq，以确定参与非更年期“黄色”甜瓜果实成熟谱的重要酶和途径，重点关注糖代谢。我们鉴定了授粉后 10 天 (DAP) 偏向的 895 个基因和 40 DAP 偏向的 909 个基因。对这些差异表达（DE）基因的 KEGG 通路富集分析表明，“激素信号转导”、“碳代谢”、“蔗糖代谢”、“内质网中的蛋白质加工”和“剪接体”是发生在瓜开发。在蔗糖代谢中，果实成熟过程中 5 个 DE 基因上调，12 个基因下调。结果表明，糖途径中的重要酶负责非更年期“黄”甜瓜的蔗糖含量和成熟特征。本研究首次在甜瓜中检测到新的 DE 基因，如转化酶抑制剂 LIKE 3 (CmINH3)、海藻糖磷酸磷酸酶 (CmTPP1) 和海藻糖磷酸合酶 (CmTPS5、CmTPS7、CmTPS9)。此外，蛋白质-蛋白质网络相互作用的结果展示了幼瓜和成熟瓜转录组的一般特征，为理解成熟提供了新的视角。