Panch phoron is a mixture of five spices containing an equal proportion of Foeniculum vulgare (fennel), Trigonella foenum-graecum Linn (fenugreek), Nigella sativa (black cumin), Cuminum cyminum (cumin) and Brassica nigra (black mustard). The mixture is commonly used in Bangladeshi cuisine and possesses many pharmacological effects. In this study, we evaluated the antinociceptive and anti-inflammatory activities of aqueous panch phoron extract (PPE) in vivo, its possible mechanism of action and phytochemical analysis by High-Performance Liquid Chromatography (HPLC). We also investigated the effect of PPE on postoperative pain in mice. HPLC was carried out using LC-20A Modular HPLC system to identify the bioactive compounds present in PPE. Five groups of Swiss albino male mice (n = 6 per group) were orally treated with 10 ml/kg of distilled water or 10 mg/kg of sodium diclofenac or three doses of PPE (100 mg/kg, 300 mg/kg, 500 mg/kg). In vivo assessment was carried out by the writhing test, tail-flick test, formalin test, and carrageenan induced paw edema test. The opioid antagonist, naloxone was used in the acetic acid test to evaluate the involvement of opioid receptors. To assess the effect of PPE in postoperative pain, mice that underwent sciatic nerve surgery were measured for the paw withdrawal latency in a hot water bath. In HPLC analysis, different types of phenolic compounds and flavonoids, including catechin hydrate, para-coumaric acid, vanillic acid, and syringic acid were detected. Treatment with PPE exhibited dose-dependent antinociceptive and anti-inflammatory activities in pain models (p < 0.05). Furthermore, naloxone did not reverse the effect of PPE in the writhing test. Mice that underwent sciatic nerve surgery showed that the paw withdrawal latency increased gradually over 7 days. Our results demonstrate that PPE has significant antinociceptive and anti-inflammatory activities and can provide significant postoperative analgesia.

中文翻译：

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一种机械方法，用于HPLC分析，panch phoron的抗伤害感受，抗炎和术后镇痛作用。

Panch phoron是五种香料的混合物，其中包含相等比例的小茴香（Feneniculum vulgare）（茴香），三角叶草（Trigonella foenum-graecum Linn）（茴香），Nigella sativa（黑孜然），小茴香（Cuminum cyminum）（小茴香）和芸苔（Brassica nigra（黑芥末））。该混合物常用于孟加拉菜，并具有许多药理作用。在这项研究中，我们评估了水性水phor蛋白提取物（PPE）在体内的抗伤害感受和抗炎活性，其可能的作用机理以及通过高效液相色谱（HPLC）进行的植物化学分析。我们还研究了PPE对小鼠术后疼痛的影响。使用LC-20A模块化HPLC系统进行HPLC，以鉴定PPE中存在的生物活性化合物。五组瑞士白化病雄性小鼠（每组n = 6）分别用10 ml / kg蒸馏水或10 mg / kg双氯芬酸钠或三剂PPE口服治疗（100 mg / kg，300 mg / kg，500毫克/公斤）。通过扭体试验，甩尾试验，福尔马林试验和角叉菜胶诱发的爪水肿试验进行体内评估。阿片拮抗剂纳洛酮用于乙酸测试，以评估阿片受体的参与。为了评估PPE在术后疼痛中的作用，对接受坐骨神经手术的小鼠在热水浴中的爪退缩潜伏期进行了测量。在HPLC分析中，检测到不同类型的酚类化合物和类黄酮，包括儿茶素水合物，对香豆酸，香草酸和丁香酸。在疼痛模型中，PPE治疗显示出剂量依赖性的抗伤害感受和抗炎活性（p <0.05）。此外，纳洛酮在扭转试验中并未逆转PPE的作用。接受坐骨神经手术的小鼠显示，爪缩回潜伏期在7天内逐渐增加。我们的结果表明，PPE具有显着的抗伤害感受和抗炎活性，并且可以提供重要的术后镇痛作用。