S100 calcium binding protein A12 (S100A12) is a member of the S100 protein family and is widely expressed in neutrophil and low expressed in lymphocytes and monocyte. However, the role of S100A12 in glioma has not yet been identified. In the present study, we carried out immunohistochemical investigation of S100A12 in 81 glioma tissues to determine the expression of S100A12 in glioma cells, and evaluate the clinical significance of S100A12 in glioma patients. Futher we knockdown the S100A12 by shRNA, and evaluated cell proliferation, cell migration and cell apoptosis by MTT, colony formation assay, transwell assay,flow cytometry assa and western blot. We found that S100A12 was upregulated in tissues of glioma patients and the expression was correlated to WHO stage and tumor size. Further, we found that knockdown S100A12 inhibits the proliferation, migration and invasion of glioma cells through regulating cell apoptosis and EMT. S100A12 plays a vital role in glioma progression, and may be an important regulatory molecule for biological behaviors of glioma cell lines.

中文翻译：

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S100钙结合蛋白A12的下调抑制神经胶质瘤细胞的生长

S100钙结合蛋白A12（S100A12）是S100蛋白家族的成员，在嗜中性粒细胞中广泛表达，而在淋巴细胞和单核细胞中则低表达。然而，尚未确定S100A12在神经胶质瘤中的作用。在本研究中，我们对81个神经胶质瘤组织中的S100A12进行了免疫组织化学研究，以确定S100A12在神经胶质瘤细胞中的表达，并评估S100A12在神经胶质瘤患者中的临床意义。此外，我们通过shRNA敲低S100A12，并通过MTT，集落形成测定，transwell测定，流式细胞仪和western blot评估细胞增殖，细胞迁移和细胞凋亡。我们发现S100A12在神经胶质瘤患者的组织中上调，其表达与WHO分期和肿瘤大小有关。此外，我们发现敲低S100A12可抑制增殖，胶质瘤细胞通过调节细胞凋亡和EMT的迁移和侵袭。S100A12在神经胶质瘤的发展中起着至关重要的作用，并且可能是神经胶质瘤细胞系生物学行为的重要调控分子。