Milk Metabotyping Identifies Metabolite Alterations in the Whole Raw Milk of Dairy Cows with Lameness,Journal of Agricultural and Food Chemistry

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Milk Metabotyping Identifies Metabolite Alterations in the Whole Raw Milk of Dairy Cows with Lameness
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-04-03 , DOI: 10.1021/acs.jafc.9b08312
Grzegorz Zwierzchowski ₁ , Guanshi Zhang ₁ , Rupasri Mandal ₂ , David S. Wishart ₂ , Burim N. Ametaj ₁

Affiliation

The objective of this study was to evaluate whether whole raw milk originating from Holstein dairy cows affected by lameness alters its composition. A total of 20 healthy control cows and 6 cows diagnosed with lameness were selected out of 100 sampled cows in a nested case control study at 2 weeks postpartum, and whole raw milk samples were collected and analyzed with direct inject/liquid chromatography–tandem mass spectrometry and nuclear magnetic resonance. In total, 168 metabolites were identified and quantified using an in-house mass spectrometry library. A total of 35 of the identified metabolites decreased versus control cows. Only two metabolites (i.e., sn-glycero-3-phosphocholine and phosphatidylethanolamine ae C42:1) were increased in the milk of lame cows. In conclusion, milk metabotyping of lame cows revealed significant changes in multiple milk components, including amino acids, lipids, and biogenic amines. Most of the milk compounds identified as altered were lowered, suggesting deflection of nutrients from the mammary gland to the host needs for healing lameness-associated pathological processes.

中文翻译：

牛奶Metabotyping识别出me足的奶牛全脂生乳中的代谢物变化

这项研究的目的是评估源自受la行影响的荷斯坦奶牛的全脂牛奶是否会改变其成分。在产后2周的巢式病例对照研究中，从100头取样的母牛中，总共选择了20头健康对照母牛和6头被诊断为me行的母牛，并收集了全脂牛奶样品并通过直接注射/液相色谱-串联质谱法进行了分析。和核磁共振。使用内部质谱库，总共鉴定并定量了168种代谢物。与对照牛相比，总共35种已鉴定的代谢产物减少。只有两个代谢物（即SNglyc脚牛的牛奶中β-甘油-3-磷酸胆碱和磷脂酰乙醇胺ae C42：1）增加。总而言之，cow牛的牛奶亚型鉴定显示出多种牛奶成分的显着变化，包括氨基酸，脂质和生物胺。鉴定为改变的大多数牛奶化合物都降低了，表明营养物质从乳腺转移到宿主，以治愈与me行相关的病理过程。

更新日期：2020-04-03

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