Formation and Degradation Tracking of a Composite Hydrogel Based on UCNPs@PDA,Macromolecules

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Formation and Degradation Tracking of a Composite Hydrogel Based on UCNPs@PDA
Macromolecules ( IF 5.1 ) Pub Date : 2020-03-30 , DOI: 10.1021/acs.macromol.0c00072
Wenna Wu ₁ , Ling Wang ₁ , Jin Yuan ₁ , Zhuo Zhang ₁ , Xiaolai Zhang ₁ , Shuli Dong ₁ , Jingcheng Hao ₁

Affiliation

The constant and noninvasive tracking of the distribution and degradation of engineered hydrogel scaffolds using fluorescent probes is considered to be one of the important research studies. Conventional fluorophores were simply mixed into hydrogels by physical doping, and they suffered from photoinstability or UV–vis light excitation, which usually led to the potential leak of the fluorescent tag and imprecise tracking results. In this article, upconversion nanoparticles, NaGdF₄:Yb³⁺,Er³⁺@NaGdF₄ (UCNPs) with near-infrared light (NIR) excitation, were synthesized and were coated with polydopamine (PDA). A biodegradable composite hydrogel OSA-I-CMCS-I-UCNPs@PDA (“I” means “linked-by”) was constructed by the UCNPs@PDA, serving as both the construction unit and NIR-excited fluorescent probe, where carboxymethyl chitosan (CMCS) was used as the cross-linker to chemically cross-link UCNPs@PDA and oxidized sodium alginate (OSA) based on dynamic covalent Schiff-base linkages. It is demonstrated that the composite hydrogels possess enhanced mechanical strength, excellent self-healing capacity, injectable performance, and good biocompatibility with the tissue. In addition, the composite hydrogels possess a deep penetrating ability from UCNPs@PDA in vitro through about 10 mm thick chicken strips. A mimetic lysozyme biodegradation test was performed for 108 h in vitro for evaluating the feasibility and accuracy of UCNPs@PDA in tracking the hydrogel degradation. The degradation signals were obtained by the decrease in fluorescence intensity, which were well consistent with the weight changes in composite hydrogels, suggesting the accuracy of the UCNPs@PDA in consecutively monitoring the hydrogel degradation in vitro. With these superior properties, the composite hydrogels are expected to be promising candidates for various biomedical fields, for example, as tissue engineering or delivery carriers in vivo.

中文翻译：

基于UCNPs @ PDA的复合水凝胶的形成与降解追踪

使用荧光探针对工程水凝胶支架的分布和降解进行持续且无创的追踪被认为是重要的研究之一。常规荧光团通过物理掺杂简单地混入水凝胶中，它们遭受光不稳定或紫外线可见光激发，这通常导致荧光标签的潜在泄漏和不精确的跟踪结果。在本文中，上转换纳米颗粒NaGdF ₄：Yb ³⁺，Er ³⁺ @NaGdF ₄合成了具有近红外光（NIR）激发的（UCNPs），并用聚多巴胺（PDA）包被。UCNPs @ PDA构建了可生物降解的复合水凝胶OSA-I-CMCS-I-UCNPs @ PDA（“ I”表示“ linked-by”），同时用作构建单元和NIR激发的荧光探针，其中羧甲基壳聚糖（CMCS）被用作基于动态共价席夫碱键的UCNPs @ PDA和氧化海藻酸钠（OSA）的化学交联剂。已证明该复合水凝胶具有增强的机械强度，优异的自愈能力，可注射的性能以及与组织的良好生物相容性。此外，复合水凝胶具有从UCNPs @ PDA体外穿透约10毫米厚的鸡条的深层穿透能力。在体外进行了模拟溶菌酶生物降解测试108小时，以评估UCNPs @ PDA在跟踪水凝胶降解中的可行性和准确性。降解信号是通过荧光强度的降低获得的，这与复合水凝胶的重量变化非常吻合，表明UCNPs @ PDA在连续监测体外水凝胶降解中的准确性。凭借这些优越的性能，复合水凝胶有望成为各种生物医学领域的有前途的候选者，例如，作为体内的组织工程或递送载体。这与复合水凝胶的重量变化非常吻合，表明UCNPs @ PDA在连续监测体外水凝胶降解中的准确性。凭借这些优越的性能，复合水凝胶有望成为各种生物医学领域的有前途的候选者，例如，作为体内的组织工程或递送载体。这与复合水凝胶的重量变化非常吻合，表明UCNPs @ PDA在连续监测体外水凝胶降解中的准确性。凭借这些优越的性能，复合水凝胶有望成为各种生物医学领域的有前途的候选者，例如，作为体内的组织工程或递送载体。

更新日期：2020-03-30

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