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Lycium barbarum polysaccharides ameliorate intestinal barrier dysfunction and inflammation through the MLCK-MLC signaling pathway in Caco-2 cells.
Food & Function ( IF 5.1 ) Pub Date : 2020-03-28 , DOI: 10.1039/d0fo00030b Wei Li 1 , Mingbo Gao 1 , Ting Han 1
Food & Function ( IF 5.1 ) Pub Date : 2020-03-28 , DOI: 10.1039/d0fo00030b Wei Li 1 , Mingbo Gao 1 , Ting Han 1
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Impairment of the intestinal barrier often occurs in inflammatory bowel diseases, and pro-inflammatory factors play a vital role in the pathogenesis of intestinal diseases. In our study, the potential protective effects of Lycium barbarum polysaccharides (LBP) against intestinal barrier dysfunction evoked by pro-inflammatory factors and its anti-inflammatory effects were investigated. Caco-2 cells were stimulated with or without tumor necrosis factor (TNF)-α in the presence or absence of LBP. Our findings showed that LBP assuaged the increase of paracellular permeability and the decrease of transepithelial electrical resistance (TER) in Caco-2 cells. In addition, LBP also prevented the secretion of pro-inflammatory markers (IL-8, IL-6, ICAM-1 and MCP-1) in TNF-α-challenged Caco-2 cells. Moreover, LBP inhibited the overexpression of tight junction (TJ) proteins (claudin-1, ZO-3, and occludin) and the increase of MLCK, pMLC, p-IκBα and NFκBp65 protein expression evoked by TNF-α was suppressed by LBP pre-incubation. This finding indicated that LBP improve TNF-α-evoked intestinal barrier dysfunction via suppressing the MLCK-MLC signaling pathway mediated by NFκB.
中文翻译：
枸杞多糖通过Caco-2细胞中的MLCK-MLC信号通路改善肠道屏障功能障碍和炎症。
在炎性肠病中常常发生肠屏障的损害，而促炎因子在肠病的发病机理中起着至关重要的作用。在我们的研究中，研究了枸杞多糖（LBP）对促炎因子引起的肠道屏障功能障碍的潜在保护作用及其抗炎作用。在有或没有LBP的情况下，用或不用肿瘤坏死因子（TNF）-α刺激Caco-2细胞。我们的研究结果表明，LBP可以缓解Caco-2细胞的副细胞通透性增加和跨上皮电阻（TER）降低。此外，LBP还阻止了TNF-α攻击的Caco-2细胞中促炎性标志物（IL-8，IL-6，ICAM-1和MCP-1）的分泌。此外，LBP可抑制紧密连接（TJ）蛋白（claudin-1，ZO-3和occludin）的过表达，而LBP预孵育可抑制TNF-α引起的MLCK，pMLC，p-IκBα和NFκBp65蛋白表达的增加。 。这一发现表明，LBP通过抑制NFκB介导的MLCK-MLC信号通路，改善了TNF-α引起的肠屏障功能障碍。
更新日期：2020-03-28
中文翻译：
枸杞多糖通过Caco-2细胞中的MLCK-MLC信号通路改善肠道屏障功能障碍和炎症。
在炎性肠病中常常发生肠屏障的损害，而促炎因子在肠病的发病机理中起着至关重要的作用。在我们的研究中，研究了枸杞多糖（LBP）对促炎因子引起的肠道屏障功能障碍的潜在保护作用及其抗炎作用。在有或没有LBP的情况下，用或不用肿瘤坏死因子（TNF）-α刺激Caco-2细胞。我们的研究结果表明，LBP可以缓解Caco-2细胞的副细胞通透性增加和跨上皮电阻（TER）降低。此外，LBP还阻止了TNF-α攻击的Caco-2细胞中促炎性标志物（IL-8，IL-6，ICAM-1和MCP-1）的分泌。此外，LBP可抑制紧密连接（TJ）蛋白（claudin-1，ZO-3和occludin）的过表达，而LBP预孵育可抑制TNF-α引起的MLCK，pMLC，p-IκBα和NFκBp65蛋白表达的增加。 。这一发现表明，LBP通过抑制NFκB介导的MLCK-MLC信号通路，改善了TNF-α引起的肠屏障功能障碍。
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