Wound infection is a serious clinical problem, and the most common infection-causing bacteria are Staphylococcus aureus and Pseudomonas aeruginosa. Angelica dahurica and Rheum officinale extract (ARE) was reported to accelerate excisional wound healing in rats. In this study, we investigated the therapeutic effects of ARE on bacterial-infected wounds. Thirty Sprague-Dawley rats were divided into three groups: normal saline (NS), ARE, and biomycin ointment (BO). Full-thickness dorsal excisions in all the rats were infected with 10⁸ colony-forming units of S. aureus; the treatments were applied once daily for 7 days. Results showed that the residual wound area in ARE group was smaller than those in NS and BO groups. TBCs on wound sites gradually decreased in ARE and BO groups. The body temperature and plasma inflammatory cytokines (TNF-α, IL-6) levels increased after bacterial infection at 24 h in all groups. After treatment, BT and inflammatory cytokines levels decreased in ARE group. Histological observations showed ARE group exhibited earlier scab formation, denser dermal granulation tissue, thicker epidermis, and more angiogenesis markers than the other groups. In conclusion, ARE accelerated wound healing in S. aureus-infected wounds. We proposed ARE exhibited potential antimicrobial and anti-inflammatory effects and stimulated angiogenesis, thus improving healing in infected wounds.

伤口感染是一个严重的临床问题，最常见的引起感染的细菌是金黄色葡萄球菌和铜绿假单胞菌。据报道当归和大黄提取物（ARE）可以促进大鼠切开伤口的愈合。在这项研究中，我们调查了ARE对细菌感染伤口的治疗作用。将30只Sprague-Dawley大鼠分为三组：生理盐水（NS），ARE和生物霉素软膏（BO）。用10 ^{8 8}个金黄色葡萄球菌集落形成单位感染所有大鼠的全层背侧切除; 每天进行一次治疗，共7天。结果表明，ARE组的残余伤口面积小于NS和BO组。ARE和BO组伤口部位的TBC逐渐减少。所有组中细菌感染后24小时，体温和血浆炎性细胞因子（TNF-α，IL-6）水平均升高。治疗后，ARE组的BT和炎性细胞因子水平降低。组织学观察显示，与其他组相比，ARE组显示出更早的sc形成，更厚的皮肤肉芽组织，更厚的表皮和更多的血管生成标记。总之，ARE加速了金黄色葡萄球菌的伤口愈合感染的伤口。我们建议ARE表现出潜在的抗微生物和抗炎作用，并刺激血管生成，从而改善感染伤口的愈合。