The shortcomings of standard plasma-separation methods limit the point-of-care application of microfluidics in clinical facilities and at the patient's bedside. To overcome the limitations of this inconvenient, laborious, and costly technique, a new plasma-separation technique and device were developed. This new separation method relies on immunological capture and filtration to exclude cells from plasma, and is convenient, easy to use, and cost-effective. Most of the RBCs can be captured and immobilized by antibody which coated in separation matrix, and residue cells can be totally removed from the sample by a commercially plasma purification membranes. A 400 µL anti-coagulated whole blood sample with 65% hematocrit (Hct) can be separated by the device in 5 min with only one pipette. Up to 97% of the plasma can be recovered from the raw blood sample with a separation efficiency at 100%. The recovery rate of small molecule compounds, proteins, and nucleic acid biomarkers is evaluated; there are no obvious differences from the centrifuge method. The results demonstrate that this method is an excellent replacement for traditional plasma preparation protocols.

中文翻译：

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基于免疫捕获和过滤的高效血浆分离器。

标准血浆分离方法的缺点限制了微流体在临床设施和患者床旁的即时护理应用。为了克服这种不便，费力和昂贵的技术的局限性，开发了一种新的等离子体分离技术和设备。这种新的分离方法依靠免疫捕获和过滤将细胞从血浆中排除，方便，易用且具有成本效益。大多数RBC可以被包被在分离基质中的抗体捕获并固定，而残留的细胞可以通过商业血浆纯化膜从样品中完全去除。仅用一根移液器即可在5分钟内用该装置分离出具有65％血细胞比容（Hct）的400 µL抗凝全血样品。可以从原血样品中回收高达97％的血浆，分离效率为100％。评估了小分子化合物，蛋白质和核酸生物标记物的回收率；与离心法没有明显区别。结果表明，该方法是传统血浆制备方案的理想替代品。