The aim of this study was to investigate the sporicidal effect of a krypton-chlorine (KrCl) excilamp against Alicyclobacillus acidoterrestris spores and to identify its inactivation mechanism compared to that with a conventional ultraviolet (UV) lamp containing mercury (Hg). The inactivation effect of the KrCl excilamp was not significantly different from that of the Hg UV lamp for A. acidoterrestris spores in apple juice despite the 222-nm of the KrCl excilamp having a higher absorption coefficient in apple juice than that of the 254-nm of the Hg UV lamp; this is because KrCl excilamps have a fundamentally greater inactivation effect than that of Hg UV lamps, which is confirmed under ideal conditions [phosphate-buffered saline (PBS)]. The inactivation mechanism analysis revealed that the KrCl excilamp induced no significant difference (P > 0.05) in DNA damage compared to that with the Hg UV lamp, while the KrCl excilamp caused significantly higher (P < 0.05) lipid peroxidation incidence and permeability change in the inner membrane of A. acidoterrestris spores than did the Hg UV lamp. Meanwhile, the KrCl excilamp did not generate significant (P > 0.05) intracellular ROS, indicating that the KrCl excilamp only causes damage through the direct absorption of UV light. In addition, after KrCl excilamp treatment for a dose of 2,011 mJ/cm² to reduce A. acidoterrestris spores in apple juice by 5-log, there were no significant (P > 0.05) changes in the quality parameters such as color (L*, a*, and b*), total phenolic compounds (TPC) and DPPH free radical scavenging activity.

IMPORTANCE Alicyclobacillus acidoterrestris spores, which have high resistance to thermal treatment and can germinate even at low pH, are very troublesome in the juice industry. Ultraviolet (UV) technology, a nonthermal treatment, can be an excellent means to control heat-resistant A. acidoterrestris spores in place of thermal treatment. However, the traditionally applied UV sources are lamps that contain mercury (Hg), which is harmful to humans and the environment; thus, there is a need to apply novel UV technology without the use of Hg. In response to this issue, excilamps, a Hg-free UV source, have been actively studied. However, no studies have been conducted applying this technique to control A. acidoterrestris spores. Therefore, the results of this study, which applied a KrCl excilamp for control of A. acidoterrestris spores and elucidated the inactivation principle, are expected to be utilized as important basic data for applying to actual industry or conducting further studies.

这项研究的目的是研究a-氯（KrCl）螯合物对酸热脂环酸芽孢杆菌的杀孢作用，并确定其灭活机理与传统的含汞（Hg）的紫外线（UV）灯相比。KrCl激发子的灭活作用与汞灯对A. acidoterrestris的灭活作用没有显着差异尽管222 nm的KrCl激发电流在苹果汁中的吸收系数要比Hg紫外灯的254 nm的吸收系数高，但苹果汁中的孢子仍然存在；这是因为在理想条件下[磷酸盐缓冲液（PBS）]可以证实，KrCl兴奋剂具有比Hg紫外线灯更大的灭活效果。灭活机理分析表明，与使用Hg紫外线灯相比，KrCl兴奋剂对DNA的损伤无明显差异（P > 0.05），而KrCl兴奋剂引起的脂质过氧化率和通透性变化显着更高（P <0.05）。酸曲霉孢子的内膜比汞紫外灯的内膜好。同时，KrCl激发电流未产生明显的P > 0.05）胞内ROS，表明KrCl激发电流仅通过直接吸收紫外线引起损害。此外，KrCl excilamp治疗剂量为2011毫焦耳/平方厘米之后²减少A.耐热菌5-日志中苹果汁孢子，没有显著（P > 0.05）质量参数，例如颜色（L *的变化，a *和b *），总酚类化合物（TPC）和DPPH自由基清除活性。

重要信息 酸热脂环酸芽孢杆菌对热处理具有很高的抵抗力，即使在低pH条件下也可以发芽，这在果汁工业中非常麻烦。紫外线（UV）技术是一种非热处理方法，可以代替热处理而成为控制耐热A. acidoterrestris孢子的绝佳手段。但是，传统上使用的紫外线源是含有汞（Hg）的灯，汞对人体和环境都有害。因此，需要在不使用汞的情况下应用新颖的紫外线技术。为了解决这个问题，已经积极研究了无汞紫外线源exilamps。但是，尚未进行将该技术用于控制酸曲霉的研究。孢子。因此，本研究，其应用了KrCl excilamp为对照组的结果A.耐热菌的孢子和阐明灭活原则，有望被用作应用于实际的行业或进行进一步研究的重要基础数据。