The extraction process of Glycyrrhiza soluble polysaccharide (GP) was optimized by RSM, a rat trauma model was established via longitudinal incision on the back skin. The effects of GP combined with microcapsule collagen on the repair of rat injury model were discussed at different levels, Based on the content of hydroxyproline at the whole animal level, the proliferation of granulation tissue stained by HE, the number of microvessels labeled by CD34, the production of collagen fibers stained by Masson, the level of phosphorylation of STAT3 protein and that of VEGF at protein level were investigated. The results showed that after the administration of GP combined with microcapsules, the content of hydroxyproline in granulation tissue increased, the proliferation of capillaries and fibroblasts in granulation tissue became active, and the number of microvessels in wound increased. The formation density of collagen fibers was uniform and orderly. GP combined with microcapsules could activate the expression of p-STAT3 and VEGF proteins and up-regulate the transcription level of VEGF mRNA and miRNA-21 genes. Furthermore, GP combined with microcapsules could accelerate wound healing and promote neovascularization.

中文翻译：

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甘草可溶性多糖复合微胶囊的制备及其在伤口修复和瘢痕抑制中的应用。

通过RSM优化了甘草可溶性多糖（GP）的提取工艺，通过在背部皮肤的纵向切口建立了大鼠创伤模型。从不同水平探讨了GP联合微囊胶原蛋白对大鼠损伤模型的修复作用，根据全动物水平羟脯氨酸的含量，HE染色肉芽组织的增殖，CD34标记的微血管的数量，研究了Masson染色的胶原纤维的产生，STAT3蛋白的磷酸化水平和VEGF在蛋白水平的磷酸化水平。结果表明，GP联合微胶囊给药后，肉芽组织中羟脯氨酸的含量增加，肉芽组织中毛细血管和成纤维细胞的增殖活跃，伤口中的微血管数量增加。胶原纤维的形成密度均匀且有序。GP结合微胶囊可以激活p-STAT3和VEGF蛋白的表达，并上调VEGF mRNA和miRNA-21基因的转录水平。此外，GP与微囊结合可加速伤口愈合并促进新血管形成。