Lactic acid (LA) fermentation was conducted with suspended and immobilized cells of an isolated Lactobacillus plantarum 23 strain using various fermentation strategies. Glucose and an alternative, relatively inexpensive carbon source - the hydrolysate of microalga Chlorella vulgaris ESP-31, were used as the carbon source. Batch fermentation using immobilized cells of L. plantarum 23 could enhance LA titer and yield by 43% and 39%, respectively, when compared with the suspended culture. Fed-batch culture integrated with in situ LA removal via ion exchange raised LA productivity by 72% by overcoming product inhibition. The highest LA productivity from glucose with PVA immobilized cells was 14.22 g/L/h, achieved under continuous operation at 50% w/v loading of immobilized beads and hydraulic retention time (HRT) of 2 h. PVA immobilized L. plantarum 23 could also use microalgal hydrolysate as the renewable carbon source, and the highest LA productivity was 9.93 g/L/h under continuous fermentation at 4 h HRT.

中文翻译：

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探索使用微藻类作为原料的聚乙烯醇固定化植物乳杆菌23增强乳酸生产的发酵策略。

使用各种发酵策略，用分离的植物乳杆菌23菌株的悬浮和固定化细胞进行乳酸（LA）发酵。葡萄糖和另一种相对便宜的碳源-微藻小球藻ESP-31的水解产物用作碳源。与悬浮培养相比，使用固定的植物乳杆菌23细胞分批发酵可分别提高LA滴度和产率39％和39％。补料分批培养与通过离子交换原位LA去除相结合，通过克服产物抑制作用将LA生产率提高了72％。葡萄糖与PVA固定化细胞的最高LA生产率为14.22 g / L / h，是在固定化磁珠负载量为50％w / v和水力停留时间（HRT）为2 h的连续操作下实现的。PVA固定L.