This study aims to unveil the effect of biosurfactant as stimulant in crude oil bioremediation. Isolated oil-degrading strain, B.subtilis RSL 2 was optimized for the maximum oil degradation and biosurfactant production using Response surface methodology. The produced biosurfactant was characterized and investigated for its effect on microbial oil degradation in two modes (a) sequential and (b) simultaneous. The strain produced 3.5 g/L of biosurfactant at pH 4.0, 25°C, using 1 g/L crude oil as the only C-source in 7 days, which was characterized as lipopeptide with a critical micelle concentration (CMC) of 0.5 g/L. The biosurfactant improved surface wettability of a hydrophobic substrate i.e. increased surface energy from 30±1 to 35±1 mJ/m². Further, the simultaneous feed of biosurfactant at 0.5 CMC enhanced oil biodegradation (72%) and biosurfactant production (5.2 g/L) by about 1.6 times than the sequential mode due to improvement in mobilization of oil thus making it more bioavailable.

这项研究旨在揭示生物表面活性剂作为刺激剂在原油生物修复中的作用。使用响应面方法对分离的石油降解菌株枯草芽孢杆菌RSL 2进行了优化，以最大程度地降解石油和生产生物表面活性剂。对生产的生物表面活性剂进行了表征，并研究了其对微生物油降解的影响，包括两种模式（a）顺序模式和（b）同时模式。该菌株在pH 4.0 4.0、25°C的条件下，在7天内使用1 g / L原油作为唯一的C源，产生了3.5 g / L的生物表面活性剂，其特征为具有0.5 g临界胶束浓度（CMC）的脂质/升 生物表面活性剂改善了疏水性基材的表面润湿性，即表面能从30±1增加到35±1 mJ / m ²。此外，由于连续性提高了油的流动性，因此在0.5 CMC下同时进料生物表面活性剂可提高油生物降解率（72％）和生物表面活性剂产量（5.2 g / L）约1.6倍，从而提高了油的生物利用度。