Mango cv. Manila kernel ethanolic extract (MKE) was assessed in vitro and in mango cv. Ataulfo fruits for antifungal activity against Colletotrichum brevisporum. An optimum MKE with a total phenolic content of 461.7 mg gallic acid equivalents/g and DPPH^• antioxidant activity of 2919.0 μmol Trolox equivalents/g was obtained by applying high intensity ultrasound, using a solid/liquid of 1/20 g/mL and a sonication time of 30 min. The major phenolic compounds in MKE were mangiferin, chlorogenic acid, myricetin, ferulic acid, quercetin, rutin and caffeic acid. In vitro studies revealed that MKE at 3 g/L produced a mycelial growth inhibition of 100% after 9 d and a spore germination of 0% after 20 h. In contrast, the recommended dosage (2 g/L) of the commercial fungicide thiabendazole achieved a mycelial growth inhibition of 67.6% and a spore germination of 51.4% for the same times. Mango fruits immersed in MKE (3 g/L) aqueous solution for 20 min and inoculated with C. brevisporum (10⁵ spores/mL) presented disease lesions of 0 mm, while when immersed in thiabendazole (2 g/L) the disease lesions were of 4.5 mm, both after 9 d. Thus, MKE may be considered as effective antifungal agent.

芒果简历 马尼拉仁乙醇提取物（MKE）在体外和芒果中进行了评估。Ataulfo水果对炭疽病菌的抑菌作用。最佳的MKE的总酚含量为461.7 mg没食子酸当量/ g，DPPH ^•抗氧化活性为2919.0μmolTrolox当量/ g，通过使用高强度的超声波，使用的固体/液体为1/20 g / mL，并进行了测定。超声处理时间为30分钟。MKE中的主要酚类化合物是芒果苷，绿原酸，杨梅素，阿魏酸，槲皮素，芦丁和咖啡酸。体外研究表明，3 g / L的MKE在9 d后产生100％的菌丝生长抑制，20 h后产生0％的孢子萌发。相反，商业剂量的杀菌剂噻菌灵的推荐剂量（2 g / L）在同一时间实现了66.7％的菌丝体生长抑制和51.4％的孢子萌发。芒果果实在MKE（3 g / L）水溶液中浸泡20分钟，并用短孢梭菌（10 ⁵孢子/ mL）接种后，病灶为0 mm，而在噻菌灵中（2 g / L）浸泡时，病灶9天后均为4.5毫米。因此，MKE可以被认为是有效的抗真菌剂。