The present study is the first report of in-situ growth and application of nanorods-flower like Co3O4 nanosorbent coated on the anodized aluminum substrate for thin film microextraction (TFME) approach. The flower like Co3O4 was successfully fabricated by conversion of Co-Al layered double hydroxide (LDH) precursor to Co3O4 using the simple calcinations process. The cheap and available aluminum foil was electrochemically anodized and used as a porous substrate. Response surface methodology (RSM) was explored for optimization step. Different acidic drugs, including: paracetamol, ibuprofen, aspirin and diclofenac were extracted from biological fluids in order to investigate the capability of the prepared sorbent. The extracted analytes were then analyzed using high performance liquid chromatography-ultraviolet detection (HPLC-UV). Under the optimized conditions, the limits of detection were between 0.2 and 1.7 µg L-1 in different selected matrices. The obtained limits of quantification were also calculated to be between 0.8 and 5.1 µg L-1 in the selected matrices. In addition the enrichment factors were also in the range of 105-169. Batch-to-batch reproducibility at 100 µg L-1 concentration level was also evaluated to be lower than 5.2% (n = 3). Finally, the method was successfully used for analysis of these compounds in the biological fluids.

中文翻译：

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以分层的双氢氧化物为模板，在阳极氧化铝基板上原位合成花状Co3O4纳米棒阵列，作为纳米吸附剂，用于酸性药物的薄膜微萃取，然后进行HPLC-UV定量。

本研究是原位生长和应用纳米棒-花状Co3O4纳米吸附剂原位生长和应用的阳极化铝基板上的薄膜微萃取（TFME）方法的报道。通过使用简单的煅烧工艺，通过将Co-Al层状双氢氧化物（LDH）前体转化为Co3O4，成功制作了类似Co3O4的花朵。廉价且可用的铝箔经过电化学阳极氧化处理，并用作多孔基材。探索了响应面方法（RSM）进行优化。从生物体液中提取了不同的酸性药物，包括对乙酰氨基酚，布洛芬，阿司匹林和双氯芬酸，以研究所制备吸附剂的能力。然后使用高效液相色谱-紫外检测（HPLC-UV）分析提取的分析物。在优化的条件下，不同选择的基质中的检出限在0.2至1.7 µg L-1之间。在选定的基质中，计算得出的定量限在0.8至5.1 µg L-1之间。另外，富集因子也在105-169的范围内。还评估了在100 µg L-1浓度水平下的批次间重现性低于5.2％（n = 3）。最终，该方法成功地用于分析生物流体中的这些化合物。还评估了在100 µg L-1浓度水平下的批次间重现性低于5.2％（n = 3）。最终，该方法成功地用于分析生物流体中的这些化合物。还评估了在100 µg L-1浓度水平下的批次间重现性低于5.2％（n = 3）。最终，该方法成功地用于分析生物流体中的这些化合物。