Understanding of the molecular basis of host cell-miRNA interactions is prerequisite to the successful application of miRNAs as potential therapeutic agents. We studied the morphological and molecular consequences of over expression of three sequence divergent miRNAs previously implicated in the mesenchymal-to-epithelial transition process (MET) in three distinct mesenchymal-like cancer cell lines. The ability of miRNAs to induce morphological changes characteristic of MET positively correlated with induced changes in the expression of genes previously implicated in the process. Variability in the responses of different mesenchymal-like cells to over expression of the same miRNAs was attributable to inherent differences in trans-regulatory profiles pre-disposing these cells to miRNA-induced MET. Collectively our results indicate that miRNA-mediated regulation of MET is a highly integrated process that is significantly modulated by the molecular background of individual cells.

了解宿主细胞与miRNA相互作用的分子基础是成功将miRNA用作潜在治疗剂的前提。我们研究了在三个不同的间充质样癌细胞系中先前涉及间充质到上皮转化过程（MET）的三个序列差异性miRNA过度表达的形态学和分子后果。miRNA诱导MET形态变化的能力与先前涉及该过程的基因表达的诱导变化正相关。不同间充质样细胞对相同miRNA过度表达的反应中的变异性归因于反调节谱中的固有差异，这些差异使这些细胞易于发生miRNA诱导的MET。