Laccase (Lac) is an environmentally friendly commercially available low-cost biocatalyst that can act on phenolic groups at room temperature using ambient air. In this study, Lac immobilized silk fibroin (SF)-based hydrogels were employed to simultaneously absorb alkaline lignin (AL) and graft AL on SF to fabricate SFAL composite hydrogels. The immobilized Lac retained highly active, and mainly catalyzed inter- and intra-molecular covalent coupling between the phenolic groups of AL and the tyrosine residues of SF. Through imparting the unique structure of lignin, the SFAL composite hydrogels exhibited improved mechanical strength and anti-ultraviolet properties over the SF-based hydrogels. By making good use of Lac and turning diffusional limitations in Lac immobilization into advantages, this study developed a new efficient approach for production of sustainable value-added materials based on silk and lignin. This study offers a promising strategy to exploit the enormous potential of these natural biopolymers and enzymes.

漆酶（Laccase）是一种环境友好的可商购的低成本生物催化剂，其可以在室温下使用环境空气作用于酚基。在这项研究中，Lac固定的丝素蛋白（SF）基水凝胶被用来同时吸收碱性木质素（AL）和在SF上的接枝AL来制造SF AL复合水凝胶。固定化的Lac保留了高活性，并且主要催化了AL的酚基和SF的酪氨酸残基之间的分子间和分子内共价偶联。通过赋予木质素独特的结构，SFAL复合水凝胶显示出比SF基水凝胶更高的机械强度和抗紫外线性能。通过充分利用Lac并将Lac固定化中的扩散限制转化为优势，本研究开发了一种新的有效方法，用于生产基于丝绸和木质素的可持续增值材料。这项研究提供了开发这些天然生物聚合物和酶的巨大潜力的有前途的策略。