Anodic Aluminum Oxide Nanopore Template-Assisted Fabrication of Nanostructured Poly(vinyl alcohol) Hydrogels for Cell Studies,ACS Applied Bio Materials

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Anodic Aluminum Oxide Nanopore Template-Assisted Fabrication of Nanostructured Poly(vinyl alcohol) Hydrogels for Cell Studies
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-03-27 , DOI: 10.1021/acsabm.0c00153
Anna Schulte ₁ , Qasim F M Alhusaini ₁ , Holger Schönherr ₁

Affiliation

The effect of systematically varied mechanical properties and nano- and microscale surface topography on the adhesion and proliferation of human pancreatic cancer cells on fibronectin-functionalized poly(vinyl alcohol) (PVA) hydrogels was studied to understand the impact of these properties of the cell microenvironment on cell attachment and spreading. The mechanical properties of PVA, as assessed by atomic force microscopy (AFM) nanoindentation, were varied by the number of freezing–thawing cycles in the physical cross-linking process used for the generation of the hydrogels. Nano- and micropatterned hydrogel surfaces exposing nanosized PVA pillars and cuboids were fabricated by replicating ordered cylindrical nanopores of anodic aluminum oxide (AAO) and poly(dimethylsiloxane) (PDMS) templates, respectively. Softer PVA hydrogels, functionalized covalently with fibronectin, showed enhanced cell adhesion and proliferation of PaTu 8988t cells in comparison to stiffer hydrogels. In addition, PaTu 8988t cells favored the nanopatterned surfaces over micropatterned and flat hydrogels.

中文翻译：

用于细胞研究的纳米结构聚（乙烯醇）水凝胶的阳极氧化铝纳米孔模板辅助制造

研究了系统变化的机械性能和纳米级和微米级表面形貌对人胰腺癌细胞在纤连蛋白功能化聚乙烯醇 (PVA) 水凝胶上的粘附和增殖的影响，以了解这些特性对细胞微环境的影响关于细胞附着和扩散。通过原子力显微镜 (AFM) 纳米压痕评估 PVA 的机械性能随用于生成水凝胶的物理交联过程中冻融循环的数量而变化。通过分别复制阳极氧化铝 (AAO) 和聚 (二甲基硅氧烷) (PDMS) 模板的有序圆柱形纳米孔来制造暴露纳米 PVA 柱和长方体的纳米和微图案水凝胶表面。更柔软的 PVA 水凝胶，与纤连蛋白共价功能化后，与较硬的水凝胶相比，PaTu 8988t 细胞的细胞粘附和增殖能力增强。此外，PaTu 8988t 细胞更喜欢纳米图案表面，而不是微图案和扁平水凝胶。

更新日期：2020-04-23

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