Developing a new colorimetric bioassay for iodide determination based on gold supported iridium peroxidase catalysts,New Journal of Chemistry

当前位置： X-MOL 学术 › New J. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Developing a new colorimetric bioassay for iodide determination based on gold supported iridium peroxidase catalysts
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020/03/27 , DOI: 10.1039/c9nj06310b
Fereshteh Amourizi _{1,

2,

3,

4} , Kheibar Dashtian _{1,

2,

3,

4} , Mehrorang Ghaedi _{1,

2,

3,

4}

Affiliation

Citrate and polyvinyl alcohol capped gold nanoparticle (Au-NPs-Cy-PVA) and iridium NPs were incorporated by a chemical reduction method and characterized by UV-Vis, DLS, SEM, EDS, XRD, and FT-IR analysis. A cost-effective and straightforward colorimetric sensor dependent on the redox reaction of 3,4-diaminotoluene biomarker (DA) with Au³⁺ ions and H₂O₂ on an Au/Ir-NP catalyst mimicking enzyme-like activity, has been manufactured for the determination of I⁻ in media of interest. At the same time, prepared Au/Ir-NPs can catalyze hydrogen peroxide (H₂O₂), which is used to reduce Au³⁺ into gold nanoparticles and oxidise 3,4-diaminotoluene biomarker. I⁻ effectively expands the catalytic activity of Au/Ir-nanoparticles to decompose H₂O₂ by the interaction between Au⁺ and Au⁰ and suppresses the oxidation of 3,4-diaminotoluene biomarker. In a framework which contains Au/Ir-NPs, H₂O₂, 3,4-diaminotoluene biomarker, and HAuCl₄, increasing the I⁻ concentration results in color changes from deep purple to yellow. In light of this principle, in this work, a novel colorimetric technique for the sensitive detection of I⁻ ions was developed. This method has a detection limit of 0.058 μM and a linear range between 0.12 to 5.42 μM. The assays are profoundly selective over other ions and can be effectively applied for the determination of iodine ions in real water samples.

中文翻译：

基于金负载的铱过氧化物酶催化剂，开发一种新的比色法测定碘化物

柠檬酸盐和聚乙烯醇封端的金纳米颗粒（Au-NPs-Cy-PVA）和铱NPs通过化学还原方法掺入并通过UV-Vis，DLS，SEM，EDS，XRD和FT-IR分析进行表征。已生产出一种经济高效且简单的比色传感器，该传感器取决于3,4-二氨基甲苯生物标记（DA）与Au ³⁺离子和H ₂ O ₂在模仿酶样活性的Au / Ir-NP催化剂上的氧化还原反应。对于我的决心^-在感兴趣的媒体。同时，制备的Au / Ir-NPs可以催化过氧化氢（H ₂ O ₂），该过氧化氢可将Au ³⁺还原为金纳米颗粒并氧化3,4-二氨基甲苯生物标记。一世⁻通过Au⁺和Au⁰之间的相互作用，有效地扩展了Au / Ir纳米颗粒分解H₂ O₂的催化活性，并抑制了3,4-二氨基甲苯生物标志物的氧化。在其中包含的Au / IR-的NP的框架，H₂ ö₂，3,4-二氨基甲苯的生物标志物，和的HAuCl₄，增加了我^-颜色变化浓度的结果从深紫为黄色。在这一原理的光，在这项工作中，I的灵敏检测的新颖比色法^-离子被开发出来。该方法的检测极限为0.058μM，线性范围为0.12至5.42μM。该测定法对其他离子具有极高的选择性，可有效地用于测定真实水样中的碘离子。

更新日期：2020-04-06

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11