Modulation of protein–graphene oxide interactions with varying degrees of oxidation,Nanoscale Advances

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Modulation of protein–graphene oxide interactions with varying degrees of oxidation
Nanoscale Advances ( IF 4.6 ) Pub Date : 2020-03-27 , DOI: 10.1039/c9na00807a
Shahid A Malik _{1,

2} , Zinia Mohanta _{2,

3} , Chandan Srivastava ₄ , Hanudatta S Atreya _{1,

2}

Affiliation

The degree of oxidation of graphene oxide (GO) has been shown to be important for its toxicity and drug-loading efficiency. However, the effect of its variations on GO–protein interaction remains unclear. Here, we evaluate the effect of the different oxidation degrees of GO on its interaction with human ubiquitin (8.6 kDa) using solution state nuclear magnetic resonance (NMR) spectroscopy in combination with other biophysical techniques. Our findings show that the interaction between the protein and the different GO samples is weak and electrostatic in nature. It involves fast dynamic exchange of the protein molecules from the surface of the GO. As the oxidation degree of the GO increases, the extent of the interaction with the protein changes. The interaction of the protein with GO can thus be modulated by tuning the degree of oxidation. This study opens up new avenues to design appropriate graphenic materials for use in various biomedical fields such as drug delivery, biomedical devices and imaging.

中文翻译：

不同氧化程度调节蛋白质-氧化石墨烯相互作用

氧化石墨烯（GO）的氧化程度已被证明对其毒性和载药效率很重要。然而，其变异对 GO-蛋白质相互作用的影响仍不清楚。在这里，我们使用溶液态核磁共振 (NMR) 光谱结合其他生物物理技术评估了 GO 的不同氧化度对其与人泛素 (8.6 kDa) 相互作用的影响。我们的研究结果表明，蛋白质与不同 GO 样品之间的相互作用本质上是微弱的静电相互作用。它涉及 GO 表面蛋白质分子的快速动态交换。随着GO氧化程度的增加，与蛋白质相互作用的程度发生变化。因此，可以通过调节氧化程度来调节蛋白质与 GO 的相互作用。这项研究开辟了设计合适的石墨烯材料用于各种生物医学领域（例如药物输送、生物医学设备和成像）的新途径。

更新日期：2020-03-27

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