Solid by-products generated in the winemaking process, can comprise valuable bioactive substances such as resveratrol and viniferin, which can be used in whole range of sectors including medicine, pharmacy, cosmetic industry etc. The changes in content of those stilbenes in extracts obtained by maceration and Soxhlet extraction were monitored using newly modified and validated high-performance liquid chromatography-mass spectrometry method which was proved to be accurate, reproducible, and efficient for their determination. The yields of individual bioactive compounds isolated from winery by-products are crucially dependent on the conditions of used extraction techniques. From this point of view, stability testing including light exposure, elevated temperature, and storage for longer time periods in the solution, represents the basis for optimizing conditions of extraction methods of resveratrol and trans-ε-viniferin. High temperature is beneficial for better release of thermally more stable stilbenes such as trans-resveratrol and trans-ε-viniferin but its application for prolonged time periods can be destructive. Light stress conditions cause the formation of otherwise unavailable cis-ε-viniferin by dimerization and photoisomerization of trans- stilbenes.

在酿酒过程中产生的固体副产物可能包含有价值的生物活性物质，例如白藜芦醇和viniferin，可用于整个领域，包括医药，制药，化妆品行业等。浸提法和索氏提取法采用新近改进和验证的高效液相色谱-质谱联用方法进行监测，事实证明该方法准确，可重复且有效。从酿酒厂副产品中分离出的单个生物活性化合物的产量关键取决于所用提取技术的条件。从这个角度出发，进行稳定性测试，包括曝光，高温以及在溶液中保存更长的时间，反式-ε-葡萄素。高温有利于更好地释放热稳定的对苯二酚，例如反式白藜芦醇和反式-ε-葡萄素，但长时间使用可能会造成破坏。光胁迫条件引起其他原因不可用的形成顺-ε-葡萄素通过二聚化和光异构化的反式-二苯乙烯。