C-reactive protein (CRP) plays an important role in inflammation detection and disease monitoring. The optical biosensor is a highly sensitive and easy detection tool. The microfluidic self-driving optical sensors were fabricated with transparent glass material and used for the enhanced surface plasmon resonance (SPR) optical detection of the model protein CRP using Au nanoparticles (AuNPs) and a sandwich immune reaction. The 3D design of the chip was devised to improve the optical coupling efficiency and enable integration with a microfluidic control and rapid detection. The array of pre-fixed antibody modified by Au nanoparticles was used to achieve rapid antigen capture and improve the optical sensitivity. The Au nanoparticle amplification approach was introduced for the SPR detection of a target protein. CRP was used as a model target protein as part of a sandwich assay. The use of Au NP measurements to detect the target signal is a threefold improvement compared to single SPR detection methods.

中文翻译：

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纳米颗粒增强型自驱动微流控生物传感器。

C反应蛋白（CRP）在炎症检测和疾病监测中起着重要作用。光学生物传感器是一种高度灵敏且易于检测的工具。微流体自动驾驶光学传感器由透明玻璃材料制成，用于使用Au纳米颗粒（AuNPs）和三明治免疫反应对模型蛋白CRP进行增强的表面等离子体共振（SPR）光学检测。芯片的3D设计旨在提高光耦合效率，并实现与微流体控制和快速检测的集成。Au纳米颗粒修饰的固定抗体阵列用于快速捕获抗原并提高光学灵敏度。引入金纳米颗粒扩增方法用于靶蛋白的SPR检测。CRP被用作模型目标蛋白，作为三明治检测的一部分。与单个SPR检测方法相比，使用Au NP测量来检测目标信号是三倍的改进。