The white-backed planthopper (WBPH) Sogatella furcifera is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an important role in the WBPH life cycle. YLS diversity and function have been extensively studied in the brown planthopper (BPH) and small brown planthopper but not in WBPH, even though a novel strategy for controlling the BPH based on suppressing YLS has been proposed. Here, using denaturing gradient gel electrophoresis, we identified 12 unique fungal sequences among YLS of WBPH, and five of them represented uncultured fungi. We then fed WBPH with rice plants treated with different fungicides [70% propineb wettable powder (WP) (PR), 70% propamocarb hydrochloride aqueous solution (AS) (PH), 25% trifloxystrobin and 50% tebuconazole water-dispersible granules (WG) (TT), 40% pyrimethanil suspension concentrate (SC) (PY), and 50% iprodione SC (IP)] and evaluated their effects on YLS abundance and WBPH survival rate. Both YLS abundance and adult WBPH survival rate were significantly decreased upon feeding fungicide-treated rice plants, and exposure to 50% IP resulted in the strongest reduction. The abundance of two Sf-YLS species (Ascomycetes symbiotes and Cla-like symbiotes) was significantly reduced upon exposure to 50% IP. The counts of Ascomycetes symbiotes, the most abundant YLS species, were also suppressed by the other fungicides tested. In conclusion, 50% IP was the most effective fungicide, reducing YLS abundance and WBPH survival rate under controlled conditions, suggesting its potential use to control WBPH.

中文翻译：

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杀菌剂会降低酵母样共生菌的含量，并降低白背飞虱Sogatella furcifera（同翅目：Delphacidae）的存活。

白背飞虱（WBPH）Sogatella furcifera是东南亚水稻中最有害的害虫之一。WBPH的脂肪体带有细胞内酵母样共生物（YLS）。YLS通过卵巢感染垂直传播给WBPH后代。YLS在WBPH生命周期中起着重要作用。尽管已经提出了一种基于抑制YLS的控制BPH的新策略，但在褐飞虱（BPH）和小褐飞虱中广泛研究了YLS的多样性和功能，但在WBPH中并未对此进行广泛研究。在这里，使用变性梯度凝胶电泳，我们在WBPH的YLS中鉴定了12个独特的真菌序列，其中5个代表未培养的真菌。然后，我们向WBPH饲喂经过不同杀菌剂处理过的水稻植株[70％的proineb可湿性粉剂（WP）（PR），70％的丙氨威盐酸盐水溶液（AS）（PH），25％的三氟氧菌酯和50％的戊唑醇水分散性颗粒（WG）（TT），40％的嘧啶酮悬浮浓缩物（SC）（PY）和50％的异丙洛酮SC（IP）]，并评估了它们对YLS丰度和WBPH存活率的影响。喂食经过杀真菌剂处理的水稻植物后，YLS丰度和成年WBPH存活率均显着降低，暴露于50％IP导致最大程度的降低。两种Sf-YLS物种的丰度（暴露于50％IP后，子囊菌共生体和类Cla类共生体明显减少。被测试的其他杀真菌剂也抑制了最丰富的YLS物种-子囊菌共生菌的计数。总之，50％IP是最有效的杀菌剂，在受控条件下降低了YLS的丰度和WBPH的存活率，表明其潜在用途可用于控制WBPH。