To address concerns regarding the potential impact of antibiotic use in animal husbandry on antibiotic resistance in humans, we conducted a greenhouse-based study examining uptake of the veterinary antibiotics oxytetracycline (OTC) and monensin (MON) by Tifton 85 Bermudagrass (T85), the most commonly grown forage grass in the southeastern U.S.A. Since oxytetracycline is used in both veterinary and human medicine, its accumulation in animal products could impact human resistance to this antibiotic. Monensin is not used in human medicine but has a high potential for accumulating in the environment. Our research examined antibiotic uptake by forage grass T85, the effect of dairy manure application on its uptake, and antibiotic retention in soil. We compared unspiked, wet dairy manure to wet dairy manure spiked with MON or OTC that was soil surface applied to pots or incorporated into soil. After 6 wk, plant stem/leaf and root tissue, as well as soil samples, were assessed for antibiotic residues using enzyme-linked immunosorbent assay (ELISA). Results confirmed Tifton 85 MON and OTC uptake. Six weeks after adding the antibiotics, the greatest plant matter OTC and MON contents were 157.9 ± 70.6 and 234.4 ± 19.6 µg kg⁻¹, respectively, and 17.6 and 369.5 µg kg⁻¹, respectively, for soil. When spiked with OTC, manure incorporation led to decreased OTC uptake by T85 tissue. Bioaccumulation of these antimicrobials in livestock and in the environment is a potential concern for animal, environmental, and human health.

中文翻译：

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Tifton 85百慕大草从施肥土壤中摄取土霉素和莫能菌素

为了解决有关畜牧业使用抗生素对人类耐药性的潜在影响的担忧，我们进行了一项基于温室的研究，研究了Tifton 85 Bermudagrass（T85）对兽用土霉素（OTC）和莫能菌素（MON）的吸收。美国东南部最常种植的饲料草由于土霉素在兽医和人类医学中均被使用，因此其在动物产品中的积累会影响人类对这种抗生素的抵抗力。莫能菌素未用于人类医学，但在环境中具有很高的积累潜力。我们的研究检查了饲草T85对抗生素的吸收，乳牛粪肥对其吸收的影响以及抗生素在土壤中的保留。我们比较了不加言语，湿乳牛粪到掺有MON或OTC的湿乳牛粪，这是将土壤表面施于盆中或掺入土壤中。6周后，使用酶联免疫吸附试验（ELISA）对植物茎/叶和根组织以及土壤样品的抗生素残留进行评估。结果证实了Tifton 85 MON和OTC的吸收。添加抗生素六周后，最大的植物中OTC和MON含量分别为157.9±70.6和234.4±19.6 µg kg^-1，分别与17.6和369.5微克千克^-1，分别为土壤。当加有OTC时，粪便掺入会导致T85组织对OTC的吸收减少。这些抗微生物剂在牲畜和环境中的生物蓄积是动物，环境和人类健康的潜在隐患。