Abstract

In freshwater ecosystems, wetlands are generally distinguished from deep-water ecosystems by 2-m water level as boundary. However, the difference of sediment microbial communities between wetlands and deep-water ecosystems is still unclear. We combined 16S rRNA gene sequencing and community metabolic prediction to compare sediment microbial communities and predicted metabolic genes of wetlands (natural and constructed wetlands) and deep-water ecosystems (river and lake) along with environmental factors in summer and autumn in Dongping Lake Basin. Results showed that the deep-water ecosystems had significantly higher community richness than the wetlands in autumn in the freshwater basin, which was mostly related to the pH of sediments. However, no significant difference in community richness was found in summer. Besides, the composition of both predicted metabolic genes and microbial communities was significantly affected by dissolved organic carbon (DOC) and dissolved oxygen (DO). The wetlands exhibited high predicted gene abundances related to xenobiotic biodegradation possibly due to the high DOC or DO level. Compared with the wetlands, most of the deep-water ecosystems exhibited high predicted gene abundances related to element (carbon, nitrogen, and sulfur) metabolism possibly due to the low DOC and DO levels in the freshwater basin. This study can expand the knowledge of ecological function distribution and detoxification mechanism of microbial communities in freshwater ecosystems.

中文翻译：

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东平湖流域湖泊，河流和湿地沉积物中微生物群落结构的差异和预测的代谢潜力。

摘要

在淡水生态系统中，通常以2米水位为界将湿地与深水生态系统区分开。然而，湿地和深水生态系统之间的沉积物微生物群落的差异仍然不清楚。我们结合16S rRNA基因测序和群落代谢预测，比较了东平湖流域夏季和秋季的沉积物微生物群落，预测了湿地（天然和人工湿地）和深水生态系统（河流和湖泊）的代谢基因以及环境因素。结果表明，深水生态系统的群落丰富度明显高于秋季淡水盆地的湿地，这主要与沉积物的pH有关。但是，在夏天，社区富裕程度没有显着差异。除了，溶解的有机碳（DOC）和溶解的氧（DO）显着影响了预测代谢基因和微生物群落的组成。湿地表现出与异种生物降解有关的高预测基因丰度，这可能是由于高DOC或DO水平所致。与湿地相比，大多数深水生态系统显示出与元素（碳，氮和硫）代谢有关的高预测基因丰度，这可能是由于淡水盆地中的DOC和DO含量较低。这项研究可以拓宽淡水生态系统中微生物群落的生态功能分布和排毒机理的知识。湿地表现出与异种生物降解有关的高预测基因丰度，这可能是由于高DOC或DO水平所致。与湿地相比，大多数深水生态系统显示出与元素（碳，氮和硫）代谢有关的高预测基因丰度，这可能是由于淡水盆地中的DOC和DO含量较低。这项研究可以拓宽淡水生态系统中微生物群落的生态功能分布和排毒机理的知识。湿地表现出与异种生物降解有关的高预测基因丰度，这可能是由于高DOC或DO水平所致。与湿地相比，大多数深水生态系统显示出与元素（碳，氮和硫）代谢有关的高预测基因丰度，这可能是由于淡水盆地中的DOC和DO含量较低。这项研究可以拓宽淡水生态系统中微生物群落的生态功能分布和排毒机理的知识。