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Response of cucumber (Cucumis sativus) to perfluorooctanoic acid in photosynthesis and metabolomics.
Science of the Total Environment ( IF 8.2 ) Pub Date : 2020-03-27 , DOI: 10.1016/j.scitotenv.2020.138257 Wenchao Du 1 , Xing Liu 2 , Lijuan Zhao 3 , Yanwen Xu 3 , Ying Yin 3 , Jichun Wu 2 , Rong Ji 3 , Yuanyuan Sun 2 , Hongyan Guo 3
Science of the Total Environment ( IF 8.2 ) Pub Date : 2020-03-27 , DOI: 10.1016/j.scitotenv.2020.138257 Wenchao Du 1 , Xing Liu 2 , Lijuan Zhao 3 , Yanwen Xu 3 , Ying Yin 3 , Jichun Wu 2 , Rong Ji 3 , Yuanyuan Sun 2 , Hongyan Guo 3
Affiliation
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A mechanistic understanding of perfluorooctanoic acid (PFOA) toxicity to plants is essential for future risk assessment of PFOA in agricultural soil. In this study, soil-grown cucumber (Cucumis sativus) was exposed to 0, 0.2, and 5 mg/kg of PFOA for 60 days. At harvest, contaminant accumulation, cucumber biomass, photosynthesis profiles and metabolites were measured. Results showed that PFOA depressed cucumber biomass and accumulated highest in leaves. Photosynthesis analysis revealed that PFOA at both doses reduced the chlorophyll contents and net photosynthesis rate of cucumber leaves. Gas chromatography-mass spectrometry-based non-targeted metabolomics revealed that PFOA induced metabolic reprogramming in cucumber leaves, including up-regulation of phenols (at 0.2 and 5 mg/kg) and down-regulation of amino acids (at 5 mg/kg), indicating disrupted nitrogen and carbon metabolism. Results revealed how PFOA represses plant growth by down-regulating photosynthetic pigments and disturbing the metabolism of carbohydroxides, phenols and amino acids. These findings provide valuable information for understanding the molecular mechanisms involved in plant responses to PFOA-induced stress.
中文翻译：
黄瓜（Cucumis sativus）在光合作用和代谢组学中对全氟辛酸的反应。
机械理解全氟辛酸（PFOA）对植物的毒性对于农业土壤中PFOA的未来风险评估至关重要。在这项研究中，将土壤种植的黄瓜（Cucumis sativus）暴露于0、0.2和5 mg / kg的PFOA中60天。在收获时，测量污染物积累，黄瓜生物量，光合作用和代谢产物。结果表明，PFOA抑制黄瓜生物量并在叶片中积累最高。光合作用分析表明，两种剂量的PFOA均降低了黄瓜叶片的叶绿素含量和净光合速率。基于气相色谱-质谱的非靶向代谢组学研究表明，PFOA诱导黄瓜叶片的代谢重编程，包括酚的上调（0.2和5 mg / kg）和氨基酸的下调（5 mg / kg） ，表明氮和碳代谢被破坏。结果揭示了PFOA如何通过下调光合色素和干扰碳水化合物，酚类和氨基酸的代谢来抑制植物的生长。这些发现为理解植物对PFOA诱导的胁迫反应的分子机制提供了有价值的信息。
更新日期：2020-03-27
中文翻译：
黄瓜（Cucumis sativus）在光合作用和代谢组学中对全氟辛酸的反应。
机械理解全氟辛酸（PFOA）对植物的毒性对于农业土壤中PFOA的未来风险评估至关重要。在这项研究中，将土壤种植的黄瓜（Cucumis sativus）暴露于0、0.2和5 mg / kg的PFOA中60天。在收获时，测量污染物积累，黄瓜生物量，光合作用和代谢产物。结果表明，PFOA抑制黄瓜生物量并在叶片中积累最高。光合作用分析表明，两种剂量的PFOA均降低了黄瓜叶片的叶绿素含量和净光合速率。基于气相色谱-质谱的非靶向代谢组学研究表明，PFOA诱导黄瓜叶片的代谢重编程，包括酚的上调（0.2和5 mg / kg）和氨基酸的下调（5 mg / kg） ，表明氮和碳代谢被破坏。结果揭示了PFOA如何通过下调光合色素和干扰碳水化合物，酚类和氨基酸的代谢来抑制植物的生长。这些发现为理解植物对PFOA诱导的胁迫反应的分子机制提供了有价值的信息。
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