Until now the prebiotic potential of pure trisaccharide raffinose on human health assessed through high-throughput sequencing remains poorly investigated. In this work, an in vitro model using human fecal inocula of two healthy volunteers (D1 and D2) was used to study the prebiotic potential of raffinose and compare it with the well-stablished and commercial prebiotic lactulose. The intestinal microbiota showed preference for raffinose as substrate showing the highest consumption value at 48 h (96.0 ± 0.9% D1 and 95.3 ± 0.7% D2). The fermentation of raffinose decreased the medium pH, the ammonia concentration and the relative amount of Proteobacteria, while increasing the total production of lactate and short chain fatty acids (129.9 ± 2.6 mmol/L D1 and 179.6 ± 0.6 mmol/L D2), CO₂ (10.8 ± 0.8 mmol/L_medium D1 and 5.2 ± 0.3 mmol/L_medium D2) and the relative amount of Bifidobacterium and Lactobacillus. This study suggests that raffinose holds potential functional properties for human health.

迄今为止，通过高通量测序评估的纯三糖棉子糖对人体健康的益生元潜力仍未得到很好的研究。在这项工作中，使用两个健康志愿者（D1和D2）的人粪便接种物体外模型研究了棉子糖的益生元潜力，并将其与成熟的商业益生元乳果糖进行了比较。肠道菌群表现出优先选择棉子糖作为底物，在48 h时消耗量最高（96.0±0.9％D1和95.3±0.7％D2）。棉子糖的发酵降低了培养基的pH值，氨浓度和变形杆菌的相对含量，同时增加了乳酸和短链脂肪酸（129.9±2.6 mmol / L D1和179.6±0.6 mmol / L D2），CO的总产量。₂（10.8±0.8 mmol / L_培养基D1和5.2±0.3 mmol / L_培养基D2）和双歧杆菌和乳酸杆菌的相对量。这项研究表明，棉子糖具有人类健康的潜在功能特性。