Cationic cross-linked polymers containing labile disulfide and boronic ester linkages for effective triple responsive DNA release.,Colloids and Surfaces B: Biointerfaces

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Cationic cross-linked polymers containing labile disulfide and boronic ester linkages for effective triple responsive DNA release.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-03-27 , DOI: 10.1016/j.colsurfb.2020.110988
Satyagopal Sahoo ₁ , Saikat Maiti ₁ , Puja Poddar ₁ , Dibakar Dhara ₁

Affiliation

Disruption of DNA carriers triggered by intracellular bio-stimulants has been broadly considered as most convenient strategy for efficient DNA delivery. In this direction, we have designed and synthesized pH, redox and ATP responsive cationic cross-linked polymers (CLPs) having disulfide and reversible boronic ester linkages. These CLPs also contain folate groups that are known for their targeting capability towards cancer cells. Biophysical studies showed that these cationic CLPs exhibited more effective DNA condensation in comparison to cationic linear polymers resulting in the formation of nano-sized polyplexes with sufficient positive zeta potentials and good colloidal stability at neutral pH (∼7.4). More interestingly, the polyplexes prepared from these CLPs have the ability to selectively release complexed DNA under conditions similar to those prevalent in cancer cells such as acidic pH, ATP rich surroundings or presence of glutathione, as revealed by ethidium bromide exclusion assay, agarose gel electrophoresis, AFM measurements, etc. Therefore, these cross-linked polymers have high potential of being effective non-viral gene delivery vehicles.

中文翻译：

含有不稳定的二硫键和硼酸酯键的阳离子交联聚合物，可有效释放三重响应性DNA。

由细胞内生物刺激物引发的DNA载体破坏已被广泛认为是有效DNA传递的最便捷策略。在这个方向上，我们设计并合成了具有二硫键和可逆硼酸酯键的pH值，氧化还原和ATP响应性阳离子交联聚合物（CLP）。这些CLP还包含叶酸基团，这些叶酸基团具有针对癌细胞的靶向能力。生物物理研究表明，与阳离子线性聚合物相比，这些阳离子CLP表现出更有效的DNA缩合，导致形成了具有足够的正Zeta电位和在中性pH值（约7.4）下具有良好的胶体稳定性的纳米级多链体。更有趣的是

更新日期：2020-03-27

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