Abstract Driven by the market demand, several synthetic and natural grafts have been proposed during the last years for tendons regeneration. The synthetic grafts, which present a better mechanical performance than the natural ones, usually fail due to the lack of biocompatibility and bioactivity. Thus, chitosan (Cs) was immobilized on polypropylene (PP) surface, previously activated by plasma treatment, in order to improve the fibroblasts' adhesion and proliferation on it. The Orange II dye method and FTIR-ATR analysis proved the successful Cs immobilization on the PP surface. It was observed by SEM and optical profilometry analysis that the Cs concentration increase leads to a surface roughness (Ra value) increase, as well as to water contact angle (C.A.) decrease at least until 2% (w/V) of Cs. Using the 2% (V/V) Cs solution concentration, according to SEM analysis and resazurin assay, the developed functionalization was well succeeded in improving fibroblasts adhesion and proliferation on PP substrates surface over 7 days of culture.

中文翻译：

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通过壳聚糖固定化聚丙烯 (PP) 的表面功能化以增强人成纤维细胞的活力

摘要 在市场需求的推动下，最近几年提出了几种合成和天然移植物用于肌腱再生。合成移植物比天然移植物具有更好的机械性能，但通常由于缺乏生物相容性和生物活性而失败。因此，壳聚糖 (Cs) 被固定在聚丙烯 (PP) 表面，预先通过等离子体处理活化，以改善成纤维细胞在其上的粘附和增殖。Orange II 染料方法和 FTIR-ATR 分析证明 Cs 成功固定在 PP 表面。通过 SEM 和光学轮廓分析观察到，Cs 浓度的增加导致表面粗糙度（Ra 值）增加，以及水接触角 (CA) 减少至少直到 Cs 的 2% (w/V)。使用 2% (V/V) Cs 溶液浓度，