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Bioactive Three-Dimensional Graphene Oxide Foam/Polydimethylsiloxane/Zinc Silicate Scaffolds with Enhanced Osteoinductivity for Bone Regeneration
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-03-27 , DOI: 10.1021/acsbiomaterials.9b01931 Yang Li 1, 2 , Xing Zhang 3 , Chengbai Dai 1, 2 , Yiming Yin 1 , Ling Gong 1 , Wenzhen Pan 1, 2 , Ruqi Huang 1, 2 , Yeyang Bu 1, 2 , Xianjiu Liao 1 , Kaijin Guo 1, 2 , Fenglei Gao 1
ACS Biomaterials Science & Engineering ( IF 5.4 ) Pub Date : 2020-03-27 , DOI: 10.1021/acsbiomaterials.9b01931 Yang Li 1, 2 , Xing Zhang 3 , Chengbai Dai 1, 2 , Yiming Yin 1 , Ling Gong 1 , Wenzhen Pan 1, 2 , Ruqi Huang 1, 2 , Yeyang Bu 1, 2 , Xianjiu Liao 1 , Kaijin Guo 1, 2 , Fenglei Gao 1
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Nanocomposite scaffold materials have shown great prospect in promoting bone integration and bone regeneration. A three-dimensional graphene oxide foam/polydimethylsiloxane/zinc silicate (GF/PDMS/ZS) scaffold for bone tissue engineering was synthesized via dip coating and hydrothermal synthesis processes, resulting in the interconnected macroporous structure. The scaffold was characterized with scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and thermogravimetric (TG) analysis. The result showed that scaffolds exhibiting a porous characteristic had organic–inorganic components similar to natural bone tissue. Moreover, the scaffolds possessed suitable pore size, high porosity, and good mechanical properties. In vitro experiments with mouse bone marrow mesenchymal stem cells (mBMSCs) revealed that the composite scaffold not only has great biocompatibility but also has the ability to induce mBMSC proliferation and preferential osteogentic differentiation. Thereafter, the expression of critical genes, ALP, RUNX2, VEGFA, and OPN, was activated. In vivo analysis of critical bone defect in rabbits demonstrated superior bone formation in defect sites in the GF/PDMS/ZS scaffold group at 12 weeks of post implantation without no significant inflammatory response. All the results validated that the GF/PDMS/ZS scaffold is a promising alternative for applications in bone regeneration.
中文翻译：
具有增强骨诱导性的生物活性三维氧化石墨烯泡沫/聚二甲基硅氧烷/硅酸锌支架
纳米复合支架材料在促进骨整合和骨再生方面显示了广阔的前景。通过浸涂和水热合成工艺合成了用于骨组织工程的三维氧化石墨烯泡沫/聚二甲基硅氧烷/硅酸锌（GF / PDMS / ZS）支架，从而形成了相互连接的大孔结构。通过扫描电子显微镜（SEM），X射线衍射（XRD），X射线光电子能谱（XPS）和热重（TG）分析对支架进行表征。结果表明，具有多孔特性的支架具有与天然骨组织相似的有机-无机成分。此外，支架具有合适的孔径，高孔隙率和良好的机械性能。体外小鼠骨髓间充质干细胞（mBMSCs）的实验表明，该复合支架不仅具有良好的生物相容性，而且还具有诱导mBMSC增殖和成骨性分化的能力。此后，激活了关键基因ALP，RUNX2，VEGFA和OPN的表达。兔子的关键骨缺损的体内分析表明，植入后12周，GF / PDMS / ZS支架组的缺损部位具有良好的骨形成，没有明显的炎症反应。所有结果都证明了GF / PDMS / ZS支架是骨再生应用的有希望的替代品。
更新日期：2020-03-27
中文翻译：
具有增强骨诱导性的生物活性三维氧化石墨烯泡沫/聚二甲基硅氧烷/硅酸锌支架
纳米复合支架材料在促进骨整合和骨再生方面显示了广阔的前景。通过浸涂和水热合成工艺合成了用于骨组织工程的三维氧化石墨烯泡沫/聚二甲基硅氧烷/硅酸锌（GF / PDMS / ZS）支架，从而形成了相互连接的大孔结构。通过扫描电子显微镜（SEM），X射线衍射（XRD），X射线光电子能谱（XPS）和热重（TG）分析对支架进行表征。结果表明，具有多孔特性的支架具有与天然骨组织相似的有机-无机成分。此外，支架具有合适的孔径，高孔隙率和良好的机械性能。体外小鼠骨髓间充质干细胞（mBMSCs）的实验表明，该复合支架不仅具有良好的生物相容性，而且还具有诱导mBMSC增殖和成骨性分化的能力。此后，激活了关键基因ALP，RUNX2，VEGFA和OPN的表达。兔子的关键骨缺损的体内分析表明，植入后12周，GF / PDMS / ZS支架组的缺损部位具有良好的骨形成，没有明显的炎症反应。所有结果都证明了GF / PDMS / ZS支架是骨再生应用的有希望的替代品。
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