当前位置: X-MOL 学术Chem. Asian J. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Devising Efficient Red-Shifting Strategies for Bioimaging: A Generalizable Donor-Acceptor Fluorophore Prototype.
Chemistry - An Asian Journal ( IF 3.5 ) Pub Date : 2020-04-08 , DOI: 10.1002/asia.202000175
Cheng Chen 1 , Chong Fang 1
Affiliation  

Long emission wavelengths, high fluorescence quantum yields (FQYs), and large Stokes shifts are highly desirable features for fluorescent probes in biological imaging. However, the current development of many fluorescent probes remains largely trial-and-error and lacks efficiency. Moreover, to achieve far-red/near-infrared emission, a significant extension in the π -conjugation is usually adopted but accompanied by other drawbacks such as fluorescence loss. In this review, we discuss an effective red-shifting strategy built upon the green fluorescent protein chromophore, which enables a synergistic tuning of both the electronic ground and excited states. This approach could shorten the path toward redder emission in comparison to the conventional intramolecular charge transfer (ICT) strategy. We envision that this spectroscopy and computation-aided strategy may advance the noncanonical fluorescent protein design and be generalized to various fluorophore scaffolds for redder emission while preserving other superior properties such as high FQYs.

中文翻译:

为生物成像设计有效的红移策略:通用的供体-受体荧光团原型。

对于生物成像中的荧光探针,长发射波长,高荧光量子产率(FQYs)和较大的斯托克斯位移是非常理想的功能。但是,当前许多荧光探针的发展仍然很大程度上是反复试验并且缺乏效率。而且,为了实现远红/近红外发射,通常采用π-共轭的显着扩展,但是伴随着其他缺点,例如荧光损失。在这篇综述中,我们讨论了一种基于绿色荧光蛋白发色团的有效红移策略,该策略可实现电子基态和激发态的协同调节。与传统的分子内电荷转移(ICT)策略相比,该方法可以缩短向红色发射的路径。
更新日期:2020-03-26
down
wechat
bug