Traditionally, structures of cytoskeletal components have been studied ex situ, that is, with biochemically purified materials. There are compelling reasons to develop approaches to study them in situ in their native functional context. In recent years, cryo‐electron tomography emerged as a powerful method for visualizing the molecular organization of unperturbed cellular landscapes with the potential to attain near‐atomic resolution. Here, we review recent works on the cytoskeleton using cryo‐electron tomography, demonstrating the power of in situ studies. We also highlight the potential of this method in addressing important questions pertinent to the field of cytoskeletal biomechanics.

中文翻译：

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细胞骨架的三维组织：冷冻电子断层扫描视角。


传统上，细胞骨架成分的结构是在异位研究的，即使用生化纯化的材料。有令人信服的理由来开发方法来在它们的本地功能环境中对它们进行现场研究。近年来，冷冻电子断层扫描成为一种强大的方法，可以可视化未受干扰的细胞景观的分子组织，并有可能获得接近原子的分辨率。在这里，我们回顾了最近使用冷冻电子断层扫描进行细胞骨架的研究，展示了原位研究的力量。我们还强调了这种方法在解决与细胞骨架生物力学领域相关的重要问题方面的潜力。