ABSTRACT Carprofen (CAR) is a non-steroidal anti-inflammatory drug commonly used to treat various musculoskeletal diseases and infections in food animals. While CAR residues in food animal tissues caused by veterinary medicine abuse are a potential danger to human health. In this study, a high-affinity monoclonal antibody (mAb) against CAR was prepared, and then an immunochromatographic strip for rapidly screening CAR in bovine muscle was developed to help enforce regulatory compliance. The visual detection limit (cut-off value) by the naked eye of the fabricated test strip was 12.5 ng g−1. Scanning by the strip reader, the IC50 of the test strip was calculated as 1.743 ng g−1, and the limit of detection (LOD, IC10) was 0.283 ng g−1. The proposed method provided an efficient tool for monitoring CAR in bovine muscle. Meantime, our work suggested great potential for the analysis of CAR residues in other food matrixes.

中文翻译：

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一种用于灵敏检测牛肌肉中卡洛芬的新型金颗粒纸传感器

摘要 Carprofen (CAR) 是一种非甾体抗炎药，常用于治疗食用动物的各种肌肉骨骼疾病和感染。而兽药滥用导致食用动物组织中的CAR残留对人类健康构成潜在威胁。在本研究中，制备了针对 CAR 的高亲和力单克隆抗体 (mAb)，然后开发了一种用于快速筛选牛肌肉中 CAR 的免疫层析试纸条，以帮助加强监管合规性。制成的试纸的肉眼视觉检测限（截止值）为 12.5 ng g-1。条形阅读器扫描，计算出试纸的IC50为1.743 ng g-1，检测限（LOD，IC10）为0.283 ng g-1。所提出的方法为监测牛肌肉中的 CAR 提供了一种有效的工具。与此同时，