Determination of benzo(a)pyrene in the exhaled breath condensate of cigarette smokers by microextraction and HPLC-UV,Analytical Methods

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Determination of benzo(a)pyrene in the exhaled breath condensate of cigarette smokers by microextraction and HPLC-UV
Analytical Methods ( IF 2.7 ) Pub Date : 2020-03-26 , DOI: 10.1039/d0ay00335b
Ayda Hatefi _{1,

2,

3,

4,

5} , Sima Alvani-Alamdari _{3,

5,

6,

7} , Elaheh Rahimpour _{3,

5,

7,

8} , Maryam Khoubnasabjafari _{3,

5,

7,

9} , Vahid Jouyban-Gharamaleki _{3,

5,

7,

10} , Abolghasem Jouyban _{3,

5,

7,

11,

12}

Affiliation

A reliable and efficient ultrasound assisted-liquid–liquid microextraction method-solidification of floating organic drop method (UA-LLME-SFOD) coupled with HPLC-UV was developed based on using low density organic solvents for extraction/determination of benzo(a)pyrene in exhaled breath condensate (EBC) as an alternative biological sample. To the best of our knowledge, this is the first report on the analysis of a polycyclic aromatic hydrocarbon in EBC samples. The effect of different experimental parameters on the extraction efficiency of the analyte was investigated in detail and the optimal conditions were established. Under these conditions, the proposed method provided a good linearity in the range of 5–100 ng mL⁻¹, low limit of detection (0.48 ng mL⁻¹), and good repeatability with % RSD below 2.1%, n = 5. The developed method was successfully used to determine benzo(a)pyrene in the EBC samples of some cigarette smokers.

中文翻译：

微萃取和HPLC-UV法测定卷烟吸烟者呼出气冷凝物中的苯并（a）py

基于低密度有机溶剂对苯并（a）py的萃取/测定，建立了可靠高效的超声辅助液-液微萃取-悬浮有机液滴固化法（UA-LLME-SFOD）结合HPLC-UV呼气中的冷凝物（EBC）作为替代生物样品。据我们所知，这是关于EBC样品中多环芳烃分析的第一份报告。详细研究了不同实验参数对分析物提取效率的影响，并确定了最佳条件。在这些条件下，建议的方法在5–100 ng mL ^-1的范围内提供了良好的线性，检测下限较低（0.48 ng mL ^-1），重复性好，相对标准偏差％低于2.1％，n =5。开发的方法成功地用于测定某些吸烟者的EBC样品中的苯并（a）re。

更新日期：2020-04-24

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