Four simple, precise, accurate, and eco-friendly spectrophotometric methods manipulating ratio spectra were used for the determination of acefylline piperazine (AC) and bromhexine hydrochloride (BR) in the presence of two dosage form additives — methylparaben (MP) and propylparaben (PP). The dual wavelength in ratio spectra (DWRS) and derivative of double divisor of ratio spectra (DD-RS) methods were used for the simultaneous determination of AC and BR. The first derivative ratio zero crossing method (¹DD) was used for the determination of BR. AC can be determined using sequential spectrophotometry, where the ratio subtraction method was applied to remove the extended spectrum of BR followed by applying the dual wave length method. The linearity of the proposed methods was investigated in the ranges 5.00–60.00 and 4.00–60.00 μg/mL for AC and BR, respectively. The selectivity of the developed methods was investigated by analyzing laboratory-prepared mixtures containing different ratios of the cited drugs and additives in addition to the analysis of their pharmaceutical dosage form. The validity of the proposed methods was further assessed using the standard addition technique. The obtained results were statistically compared with those obtained by the reported method, showing no significant difference with respect to their accuracy and precision at p = 0.05.

中文翻译：

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剂量形式添加剂存在下经验证的环保型分光光度法测定乙酰苯胺哌嗪和盐酸溴己定

在两种剂型添加剂对羟基苯甲酸甲酯（MP）和对羟基苯甲酸丙酯（PP）存在的情况下，使用四种简单，精确，准确和环保的分光光度法操作比率光谱法测定乙酰基哌嗪（AC）和盐酸溴己定（BR） ）。比率光谱中的双波长（DWRS）和比率光谱的双除数（DD-RS）方法用于同时测定AC和BR。一阶导数比过零法（¹DD）用于测定BR。可以使用顺序分光光度法确定AC，其中使用比率减法去除BR的扩展光谱，然后应用双波长方法。对于AC和BR，所研究方法的线性分别在5.00-60.00和4.00-60.00μg/ mL范围内进行研究。除分析其药物剂型外，还通过分析实验室制备的混合物来研究所开发方法的选择性，所述混合物包含不同比例的所述药物和添加剂。使用标准添加技术进一步评估了所提出方法的有效性。将获得的结果与通过报告的方法获得的结果进行统计比较，