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Modification of the Method of Spectrophotometric Determination of Chlorophyll A in the Suspended Matter of Water Bodies
Journal of Applied Spectroscopy ( IF 0.8 ) Pub Date : 2020-03-26 , DOI: 10.1007/s10812-020-00965-9
R. Z. Kovalevskaya , H. A. Zhukava , B. V. Adamovich

This article describes a modification of the spectrophotometric method for the determination of chlorophyll a in seston (suspended matter), which is convenient for hydroecological monitoring. It is proposed to use nuclear filters, as they have a number of advantages over fiberglass and bulk filters. An important difference of our approach from the standard methods is the use of the short-term (up to 30 min) drying of nuclear filters with seston in a desiccator at a temperature of 50–55oC immediately after the suspended matter is deposited on the filter. A comparative analysis of the standard methodology and the proposed modification for assessing the content of chlorophyll and phaeopigments in laboratory cultures of algae (Euglena gracilis, Chlorella vulgaris) and natural plankton from a natural reservoir was carried out.

中文翻译:

分光光度法测定水体悬浮物中叶绿素A的方法的改进

本文介绍了一种分光光度法测定血清中(悬浮物)叶绿素a的方法的改进方法,该方法便于进行水生态学监测。提议使用核过滤器,因为它们比玻璃纤维和散装过滤器具有许多优点。我们的方法与标准方法的一个重要区别是,将悬浮物沉积在过滤器上之后,立即在50-55oC的温度下短期(最多30分钟)将带有核子的核过滤器与干燥器一起干燥。对标准方法和拟议的修改方法进行了比较分析,以评估天然水库中藻类(Euglena gracilis,Chlorella vulgaris)和天然浮游生物的实验室培养物中的叶绿素和phaeopigment的含量。
更新日期:2020-03-26
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